High-throughput screening based on small-molecule microarrays and OI-RD microscope

被引：0

作者：

Zhu, Chenggang ^{[1
]}

Fei, Yiyan ^{[1
]}

Zhu, Xiangdong ^{[2
]}

机构：

[1] Fudan Univ, Dept Opt Sci & Engn, Shanghai, Peoples R China

[2] Univ Calif Davis, Dept Phys, Davis, CA USA

来源：

7TH IEEE INTERNATIONAL NANOELECTRONICS CONFERENCE (INEC) 2016 | 2016年

关键词：

high-throughput screening; surface chemistry; small-molecule microarrays; label-free technologies;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We developed a novel high-throughput screening platform based on small-molecule microarrays (SMMs) and oblique-incidence reflectivity difference (OI-RD) microscope. OI-RD microscope is able to monitor tens of thousands of biomolecular interaction in a single experiment without the need to label any biomolecule. We also developed surface chemistry to prepare SMMs which is able to immobilize compounds with any nucleophilic residues on phenyl-isocyanate functionalized glass slides with high efficiencies. By using OI-RD microscope and SMMs with 3,375 bioactive compounds, we screened target proteins in high-throughput and label-free mode and we found hits for respective target protein. The novel high-throughput screening platform enables target proteins with unknown structure and/ or unknown function to be effectively screened.

引用

页数：2

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