Production of alkali tolerant cellulase free xylanase in high levels by Bacillus pumilus SV-205

被引：42

作者：

Nagar, Sushi ^{[1
]}

Mittal, Anuradha ^{[1
]}

Kumar, Davender ^{[1
]}

Gupta, Vijay Kumar ^{[1
]}

机构：

[1] Kurukshetra Univ, Dept Biochem, Kurukshetra 136119, Haryana, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2012年 / 50卷 / 02期

关键词：

Xylanase; Submerged fermentation; Bacillus pumilus; Wheat bran; Alkali stable; THERMOSTABLE XYLANASE; ENHANCED PRODUCTION; SOLID-STATE; PURIFICATION; FERMENTATION; OPTIMIZATION; WHEAT; ASH;

D O I：

10.1016/j.ijbiomac.2011.12.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The fermentation conditions were optimized for hyper production of xylanase from Bacillus pumilus SV-205. The bacterium secretes high levels (7382.7 +/- 1200 IU/mL) of cellulase-free xylanase using wheat bran led to 21.63 fold increase in activity. A combination of yeast extract and peptone stimulated highest xylanase production (2448.0 IU/mL) as compared to other combinations. The most important characteristic of the enzyme is its high pH stability (100%) over a broad pH range of 6-11 for 24 h. Thermostability studies revealed that enzyme retained 65% activity after an incubation of 2 h at 60 degrees C. The level of production is remarkable as compared to earlier reports. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：414 / 420

页数：7

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