Targeting vascular endothelium with avidin microbubbles

被引:72
作者
Korpanty, G
Grayburn, PA
Shohet, RV
Brekken, RA
机构
[1] Univ Texas, SW Med Ctr, Hamon Ctr Therapeut Oncol Res, Dept Surg, Dallas, TX 75390 USA
[2] Univ Texas, SW Med Ctr, Hamon Ctr Therapeut Oncol Res, Dept Pharmacol, Dallas, TX 75390 USA
[3] Univ Texas, SW Med Sch, Hamon Ctr Therapeut Oncol Res, Dept Surg, Dallas, TX 75390 USA
[4] Univ Texas, SW Med Sch, Hamon Ctr Therapeut Oncol Res, Dept Cardiol, Dallas, TX 75390 USA
[5] Univ Texas, SW Med Sch, Div Cardiol, Dallas, TX 75390 USA
[6] Baylor Univ, Med Ctr, Baylor Heart & Vasc Inst, Dallas, TX USA
关键词
contrast ultrasound; targeted microbubbles; vascular endothelium; molecular imaging; avidinbiotin system;
D O I
10.1016/j.ultrasmedbio.2005.06.001
中图分类号
O42 [声学];
学科分类号
070206 ; 082403 ;
摘要
Targeting microbubbles (MBs) to specific vascular beds enables contrast ultrasound to be used for molecular imaging. There are several methods for attaching targeting moieties to the surface of MBs. In the present study, we demonstrate that avidin (Av) can be incorporated into the shelf of perfluorocarbon -exposed sonicated dextrose albumin (PESDA) MBs (Av-PESDA-MBs) and serve as an anchor that links Av-PESDA-MBs to biotinylated monoclonal antibodies (mAbs). This novel linking strategy was used to conjugate Av-PESDA-MBs to mAbs specific for endoglin (CD105) or a control TgG. MBs targeted to CD105 specifically bound to endothelial cells, but not to fibroblasts, in vitro but Av-PESDA-MBs conjugated with the control IgG did not specifically target either cell type. We conclude that Av-PESDA-MBs represent a novel and attractive tool to conjugate MRs with biotinylated mAbs for the purposes of vascular targeting and molecular imaging. (E-mail: rolf.brekken@utsouthwestern.edu) (c) 2005 World Federation for Ultrasound in Medicine & Biology.
引用
收藏
页码:1279 / 1283
页数:5
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