Effects of different concentrations of enterotoxigenic and verotoxigenic E. coli on boar sperm quality

被引:73
作者
Bussalleu, E. [1 ]
Yeste, M. [2 ]
Sepulveda, L. [1 ]
Torner, E. [1 ]
Pinart, E. [1 ]
Bonet, S. [1 ]
机构
[1] Univ Girona, Inst Food & Agr Technol, Dept Biol, Girona 17071, Catalonia, Spain
[2] Autonomous Univ Barcelona, Fac Vet Med, Dept Anim Med & Surg, Unit Anim Reprod, Bellaterra 08193, Catalonia, Spain
关键词
Boar; Semen; ETEC; VTEC; Sperm quality; PCR; EXTENDED PORCINE SEMEN; ESCHERICHIA-COLI; HUMAN SPERMATOZOA; BACTERIAL-CONTAMINATION; GENITAL-TRACT; MOTILITY; INSEMINATION; NUMBERS; GENES;
D O I
10.1016/j.anireprosci.2011.07.018
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The presence of bacteria in boar semen causes economic losses in artificial insemination (AI) centers, as a consequence of alterations on boar sperm quality. For this reason, the effects of different concentrations of enterotoxigenic Escherichia coli (ETEC) and verotoxigenic E. coli (VTEC) on boar sperm quality were determined in this study, by conducting two experiments. The first one consisted of assessing these effects on boar sperm quality after incubating the inoculated doses at 37 degrees C for a 96-h period, whereas the second inoculated doses were stored at 15 degrees C during 11 days. In both experiments, the infective concentrations ranged from 10(8) cfu mL(-1) to 10(2) cfu mL(-1); the negative control being a non-inoculated dose. Twenty-four hours after inoculation, we checked by PCR for the presence of bacteria in all tubes. Sperm quality (sperm motility, sperm viability and sperm morphology) was assessed at 24 h, 48 h, 72 h and 96 h after inoculations in the first experiment (37 degrees C), and after 3, 5, 7, 9 and 11 days in the second (15 degrees C). Whereas no changes were observed in sperm morphology in both experiments, the percentages of progressive motile spermatozoa dramatically diminished after 24 h of incubation at 37 degrees C, the effect being more detrimental at the highest infective concentration of microbes. Moreover, a significant decrease in the percentage of viable spermatozoa in the tube inoculated with the highest concentration (10(8) cfu mL(-1)) was detected after 24 h of incubating contaminated doses at 37 degrees C. After 48 h of incubation, the presence of infective concentrations of ETEC and VTEC from 10(8) cfu mL(-1) to 10(3) cfu mL(-1) resulted in a significant diminution in the percentage of viable spermatozoa. These results suggest that ETEC and VTEC PCR analyses should be done in doses destined for AI to minimize the use of doses with diminished sperm quality due to the presence of bacteria and to avoid the potential spread of infective diseases. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:176 / 182
页数:7
相关论文
共 28 条
[1]   SOME OBSERVATIONS ON THE AEROBIC BACTERIAL-FLORA OF THE GENITAL-TRACT OF THE DOG AND BITCH [J].
ALLEN, WE ;
DAGNALL, GJR .
JOURNAL OF SMALL ANIMAL PRACTICE, 1982, 23 (06) :325-335
[2]   Sanitary procedures for the production of extended semen [J].
Althouse, G. C. .
REPRODUCTION IN DOMESTIC ANIMALS, 2008, 43 :374-378
[3]   Bacteriospermia in extended porcine semen [J].
Althouse, GC ;
Lu, KG .
THERIOGENOLOGY, 2005, 63 (02) :573-584
[4]   Field investigations of bacterial contaminants and their effects on extended porcine semen [J].
Althouse, GC ;
Kuster, CE ;
Clark, SG ;
Weisiger, RM .
THERIOGENOLOGY, 2000, 53 (05) :1167-1176
[5]   IS THE SPERM BACTERIAL RATIO A DETERMINING FACTOR IN IMPAIRMENT OF SPERM MOTILITY - AN INVITRO STUDY IN MAN WITH ESCHERICHIA-COLI [J].
AUROUX, MR ;
JACQUES, L ;
MATHIEU, D ;
AUER, J .
INTERNATIONAL JOURNAL OF ANDROLOGY, 1991, 14 (04) :264-270
[6]  
BJURSTROM L, 1992, AM J VET RES, V53, P670
[7]  
Bolarin Guillen A., 2011, AV TECNOL PORC, VVIII, P20
[8]   Development of a protocol for multiple staining with fluorochromes to assess the functional status of boar spermatozoa [J].
Bussalleu, E ;
Pinart, E ;
Yeste, M ;
Briz, M ;
Sancho, S ;
Garcia-Gil, N ;
Badia, E ;
Bassols, J ;
Pruneda, A ;
Casas, I ;
Bonet, S .
MICROSCOPY RESEARCH AND TECHNIQUE, 2005, 68 (05) :277-283
[9]  
DANOWSKI KM, 1999, THESIS TIERARZTLICHE
[10]   Influence of Escherichia coli on motility parameters of human spermatozoa in vitro [J].
Diemer, T ;
Weidner, W ;
Michelmann, HW ;
Schiefer, HG ;
Rovan, E ;
Mayer, F .
INTERNATIONAL JOURNAL OF ANDROLOGY, 1996, 19 (05) :271-277