Expression of arabidopsis GAI in transgenic rice represses multiple gibberellin responses

被引:83
作者
Fu, XD [1 ]
Sudhakar, D [1 ]
Peng, JR [1 ]
Richards, DE [1 ]
Christou, P [1 ]
Harberd, NP [1 ]
机构
[1] John Innes Ctr, Norwich NR4 7UH, Norfolk, England
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1105/tpc.13.8.1791
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bioactive gibberellins (GAs) are essential endogenous regulators of plant growth. GA signaling is mediated via GAI, a nuclear member of the GRAS family of plant transcription factors. Previous experiments have suggested that GAI is a GA-derepressible repressor of plant growth. Here we test this hypothesis by examining the effects of the expression of Arabidopsis GAI in transgenic Basmati rice. High-level expression of GAI caused dwarfism and reduced GA responses, and the strength of this effect was correlated with the level of transgene expression. In particular, the expression of GAI abolished the GA-mediated induction of rice aleurone alpha -amylase activity, thus implicating GAI orthologs in the well-characterized cereal aleurone GA response. The GA derepressible repressor model predicts that high-level expression of GAI should confer dwarfism, and these observations are consistent with this prediction.
引用
收藏
页码:1791 / 1802
页数:12
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