Short report:: Detection of Orientia tsutsugamushi in clinical samples by quantitative real-time polymerase chain reaction

被引：38
作者：
Singhsilarak, T
Leowattana, W
Looareesuwan, S
Wongchotigul, V
Jiang, J
Richards, AL
Watt, G
机构：
[1] Mahidol Univ, Fac Trop Med, Dept Clin Trop Med, Bangkok 10400, Thailand
[2] Mahidol Univ, Fac Trop Med, Dept Microbiol, Bangkok 10400, Thailand
[3] USN, Med Res Ctr, Rickettsial Dis Dept, Silver Spring, MD 20910 USA
[4] Univ Hawaii, Dept Med, AIDS Clin Res Program, Honolulu, HI 96822 USA
来源：
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2005年 / 72卷 / 05期
关键词：
D O I：
10.4269/ajtmh.2005.72.640
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Orientia tsutsugamushi infection causes scrub typhus, a common zoonosis of rural Asia. Orientia tsutsugamushi was recently detected by a real-time quantitative polymerase chain reaction (qPCR) assay in animal specimens. We evaluated the same qPCR assay in specimens obtained from patients with serologically proven scrub typhus infections. The 47-kDa qPCR assay was more sensitive than was mouse inoculation; it was reactive in whole blood specimens from all 10 isolate-positive patients and in 7 of 17 isolate-negative individuals (P = 0.003, Fisher's two-tailed exact test). As few as 1,076 O. tsutsugamushi copies/mu L were detected in whole blood. Four of 7 sera from isolate-proven scrub typhus infections were also reactive by qPCR. The assay was unreactive in all 12 individuals without scrub typhus infection. This is the first demonstration of a sensitive and specific real-time qPCR assay for human scrub typhus infection.
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收藏
页码：640 / 641
页数：2
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