Strategies for prokaryotic expression of eukaryotic membrane proteins

被引：46

作者：

Laage, R ^{[1
]}

Langosch, D ^{[1
]}

机构：

[1] Heidelberg Univ, Dept Neurol, D-69120 Heidelberg, Germany

来源：

TRAFFIC | 2001年 / 2卷 / 02期

关键词：

expression; synaptobrevin; syntaxin; SNARE; transmembrane segment; E; coli;

D O I：

10.1034/j.1600-0854.2001.020204.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

High-level heterologous expression of integral membrane proteins at full-length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consisting of a modular fusion moiety based on nuclease A from Staphylococcos aureus are presented.

引用

页码：99 / 104

页数：6

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