Effect of Pulsed Electric Fields (PEF) on Accumulation of Magnesium in Lactobacillus rhamnosus B 442 Cells

被引：13

作者：

Goral, Malgorzata ^{[1
]}

Pankiewicz, Urszula ^{[1
]}

机构：

[1] Univ Life Sci, Fac Food Sci & Biotechnol, Dept Anal & Food Qual Assessment, Skromna St 8, PL-20704 Lublin, Poland

来源：

JOURNAL OF MEMBRANE BIOLOGY | 2017年 / 250卷 / 05期

关键词：

PEF; Magnesium; Lactobacillus rhamnosus; Bioaccumulation; SACCHAROMYCES-CEREVISIAE CELLS; LACTIC-ACID BACTERIA; METABOLIC-ACTIVITY; PLANTARUM; 564; HEAVY-METALS; ZINC IONS; GROWTH; ELECTROPORATION; OPPORTUNITIES; OPTIMIZATION;

D O I：

10.1007/s00232-017-9986-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to determine the effect of pulsed electric fields (PEF) on accumulation of magnesium ions in Lactobacillus rhamnosus B 442 cells. Under optimized conditions, this is, on 15 min exposure of the 20 h grown culture to PEF of the 2.0 kV/cm and 20 A mu s pulse width at concentration 400 mu g Mg2+/mL medium, accumulation of magnesium in the biomass reached maximum 4.28 mg/g d.m. Optimization of PEF parameters caused an increase of magnesium concentration in the cells by 220% in comparison to the control not treated with PEF. Bacterial cell biomass enriched with Mg2+ may be an alternative for pharmacological supplementation applied in deficiency of this cation.

引用

页码：565 / 572

页数：8

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