The fluorescent reaction between berberine and DNA and the fluorometry of DNA

被引：29

作者：

Li, WY ^{[1
]}

Lu, ZH ^{[1
]}

机构：

[1] SE Univ, Natl Lab Mol & Biomol Elect, Nanjing 210096, Peoples R China

来源：

MICROCHEMICAL JOURNAL | 1998年 / 60卷 / 01期

关键词：

D O I：

10.1006/mchj.1998.1633

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Determination of DNA with berberine(BER) as a fluorescent probe was developed, based on the large fluorescence enhancement of BER in the presence of DNA and the poor fluorescence yield of BER in the absence of DNA. The calibration graph was linear in the range 0-20 mu g/ml CT DNA. The Limit of detection was 12 ng/ml CT DNA. (C) 1998 Academic Press.

引用

页码：84 / 88

页数：5

共 13 条

[11] FLUOROMETRIC ASSAY USING DIMERIC DYES FOR DOUBLE-STRANDED AND SINGLE-STRANDED-DNA AND RNA WITH PICOGRAM SENSITIVITY [J].

RYE, HS ;

DABORA, JM ;

QUESADA, MA ;

MATHIES, RA ;

GLAZER, AN .

ANALYTICAL BIOCHEMISTRY, 1993, 208 (01) :144-150

[12] FLUOROMETRIC QUANTITATION OF SINGLE-STRANDED-DNA - A METHOD APPLICABLE TO THE TECHNIQUE OF ALKALINE ELUTION [J].

STOUT, DL ;

BECKER, FF .

ANALYTICAL BIOCHEMISTRY, 1982, 127 (02) :302-307

[13] FLUORESCENCE CHARACTERISTICS OF PURINES, PYRIMIDINES, AND THEIR DERIVATIVES - MEASUREMENT OF GUANINE IN NUCLEIC ACID HYDROLYZATES [J].

UDENFRIEND, S ;

ZALTZMAN, P .

ANALYTICAL BIOCHEMISTRY, 1962, 3 (01) :49-&

← 1 2 →