The fluorescent reaction between berberine and DNA and the fluorometry of DNA

被引：29

作者：

Li, WY ^{[1
]}

Lu, ZH ^{[1
]}

机构：

[1] SE Univ, Natl Lab Mol & Biomol Elect, Nanjing 210096, Peoples R China

来源：

MICROCHEMICAL JOURNAL | 1998年 / 60卷 / 01期

关键词：

D O I：

10.1006/mchj.1998.1633

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Determination of DNA with berberine(BER) as a fluorescent probe was developed, based on the large fluorescence enhancement of BER in the presence of DNA and the poor fluorescence yield of BER in the absence of DNA. The calibration graph was linear in the range 0-20 mu g/ml CT DNA. The Limit of detection was 12 ng/ml CT DNA. (C) 1998 Academic Press.

引用

页码：84 / 88

页数：5

共 13 条

[1] FLUORESCENCE REACTION OF TERBIUM(III) WITH NUCLEIC-ACIDS IN THE PRESENCE OF PHENANTHROLINE [J].

CI, YX ;

LI, YZ ;

CHANG, WB .

ANALYTICA CHIMICA ACTA, 1991, 248 (02) :589-594

[2] SELECTIVE DETERMINATION OF DNA BY ITS ENHANCEMENT EFFECT ON THE FLUORESCENCE EU3+-TETRACYCLINE COMPLEX [J].

CI, YX ;

LI, YZ ;

LIU, XJ .

ANALYTICAL CHEMISTRY, 1995, 67 (11) :1785-1788

[3] USE OF THE FLUORESCENT INTERCALATING DYES POPO-3, YOYO-3 AND YOYO-1 FOR ULTRASENSITIVE DETECTION OF DOUBLE-STRANDED DNA SEPARATED BY CAPILLARY ELECTROPHORESIS WITH HYDROXYPROPYLMETHYL CELLULOSE AND NON-CROSS-LINKED POLYACRYLAMIDE [J].

FIGEYS, D ;

ARRIAGA, E ;

RENBORG, A ;

DOVICHI, NJ .

JOURNAL OF CHROMATOGRAPHY A, 1994, 669 (1-2) :205-216

[4] USE OF ANTI-TUMOUR ANTIBIOTICS FOR SIMPLE QUANTITATIVE ASSAYS FOR DNA [J].

HILL, BT .

ANALYTICAL BIOCHEMISTRY, 1976, 70 (02) :635-638

[5] SIMPLE AND RAPID FLUORIMETRIC METHOD FOR DNA MICROASSAY [J].

KAPUSCINSKI, J ;

SKOCZYLAS, B .

ANALYTICAL BIOCHEMISTRY, 1977, 83 (01) :252-257

[6] A MODIFIED SPECTROFLUOROMETRIC ASSAY FOR DNA USING ADRIAMYCIN [J].

KENNEDY, DG ;

NELSON, J ;

VANDENBERG, HW ;

MURPHY, RF .

ANALYTICAL BIOCHEMISTRY, 1987, 167 (01) :124-127

[7]

KISSANE JM, 1958, J BIOL CHEM, V233, P184

[8] SIMPLE, RAPID, AND SENSITIVE DNA ASSAY PROCEDURE [J].

LABARCA, C ;

PAIGEN, K .

ANALYTICAL BIOCHEMISTRY, 1980, 102 (02) :344-352

[9] ONE-STEP FLUOROMETRIC MICROASSAY OF DNA IN PROCARYOTES [J].

LEGROS, M ;

KEPES, A .

ANALYTICAL BIOCHEMISTRY, 1985, 147 (02) :497-502

[10] ETHIDIUM DIMER - NEW REAGENT FOR THE FLUORIMETRIC DETERMINATION OF NUCLEIC-ACIDS [J].

MARKOVITS, J ;

ROQUES, BP ;

LEPECQ, JB .

ANALYTICAL BIOCHEMISTRY, 1979, 94 (02) :259-264

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