Applications of one-bead one-compound combinatorial libraries and chemical microarrays in signal transduction research

被引:83
作者
Lam, KS
Liu, RW
Miyamoto, S
Lehman, AL
Tuscano, JM
机构
[1] Univ Calif Davis, Ctr Canc, Div Hematol Oncol, Sacramento, CA 95817 USA
[2] Univ Calif Davis, Dept Internal Med, Sacramento, CA 95817 USA
关键词
D O I
10.1021/ar0201299
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The "one-bead-one-compound" (OBOC) combinatorial library method synthesizes millions of random compounds such that each bead displays only one compound. Bead libraries are screened, and positive beads are isolated for structure analysis. Peptide substrates and inhibitors of protein kinases, and peptide ligands for cell surface receptors have been identified using this method. A novel encoding strategy for OBOC libraries has been developed to identify peptidomimetic and small-molecule ligands that specifically interact with cellular proteins. These ligands will be tested for their effects on cell signaling and used to construct chemical microarrays for further characterization of ligand-protein interactions.
引用
收藏
页码:370 / 377
页数:8
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