E1AF degradation by a ubiquitin-proteasome pathway

被引:16
|
作者
Takahashi, A
Higashino, F
Aoyagi, M
Yoshida, K
Itoh, M
Kobayashi, M
Totsuka, Y
Kohgo, T
Shindoh, M
机构
[1] Hokkaido Univ, Grad Sch Dent Med, Dept Oral Pathobiol Sci, Kita Ku, Sapporo, Hokkaido 0608586, Japan
[2] Sapporo Med Univ, Dept Biol, Sch Med, Chuo Ku, Sapporo, Hokkaido 0608556, Japan
[3] Hokkaido Univ, Div Canc Pathobiol, Inst Med Genet, Kita Ku, Sapporo, Hokkaido 0600815, Japan
关键词
E1AF; ETS; PEA3; proteasome; ubiquitin; degradation;
D O I
10.1016/j.bbrc.2004.12.045
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
E1AF is a member of the ETS family of transcription factors. In mammary tumors. overexpression of E1AF is associated with tumorigenesis, but E1AF protein has hardly been detected and its degradation mechanism is not yet clear. Here we show that E1AF protein is stabilized by treatment with the 26S protease inhibitor MG132. We found that E1AF was modified by ubiquitin through the C-terminal region and ubiquitinated E1AF aggregated in nuclear dots, and that the inhibition of proteasome-activated transcription from E1AF target promoters. These results suggest that E1AF is degraded via the ubiquitin-proteasome pathway. which has some effect on E1AF function. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:575 / 580
页数:6
相关论文
共 50 条
  • [41] Mechanism and regulation of MAT alpha 2 degradation by the ubiquitin-proteasome pathway
    Hochstrasser, M
    MOLECULAR BIOLOGY OF THE CELL, 1997, 8 : 2057 - 2057
  • [42] Degradation of the inducible cAMP early repressor (ICER) by the ubiquitin-proteasome pathway
    Folco, EJ
    Koren, G
    BIOCHEMICAL JOURNAL, 1997, 328 : 37 - 43
  • [43] Enzymatic Blockade of the Ubiquitin-Proteasome Pathway
    Ernst, Robert
    Claessen, Jasper H. L.
    Mueller, Britta
    Sanyal, Sumana
    Spooner, Eric
    van der Veen, Annemarthe G.
    Kirak, Oktay
    Schlieker, Christian D.
    Weihofen, Wilhelm A.
    Ploegh, Hidde L.
    PLOS BIOLOGY, 2011, 9 (03)
  • [44] Auxin signaling involves regulated protein degradation by the ubiquitin-proteasome pathway
    Ward, SP
    Estelle, M
    JOURNAL OF PLANT GROWTH REGULATION, 2001, 20 (03) : 265 - 273
  • [45] Ubiquitin-proteasome pathway in reproductive tissues
    Wang, HM
    Zhu, C
    PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2002, 29 (01) : 31 - 34
  • [46] Calsenilin is degraded by the ubiquitin-proteasome pathway
    Jang, Changhwan
    Choi, Jin-Kyu
    Kim, EunYoung
    Park, Eun-Su
    Wasco, Wilma
    Buxbaum, Joseph D.
    Kim, Yong-Sun
    Choi, Eun-Kyoung
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2011, 405 (02) : 180 - 185
  • [47] The ubiquitin-proteasome pathway and plant development
    Moon, J
    Parry, G
    Estelle, M
    PLANT CELL, 2004, 16 (12): : 3181 - 3195
  • [48] The role of the ubiquitin-proteasome pathway in apoptosis
    Robert Z Orlowski
    Cell Death & Differentiation, 1999, 6 : 303 - 313
  • [49] PROTEIN DEGRADATION THROUGH THE UBIQUITIN-PROTEASOME PATHWAY IS NECESSARY FOR PROPLATELET PRODUCTION
    Machlus, Kellie
    Soussou, Thomas
    Italiano, Joseph
    AMERICAN JOURNAL OF HEMATOLOGY, 2016, 91 (09) : E399 - E399
  • [50] Regulation of bovine papillomavirus replicative helicase E1 by the ubiquitin-proteasome pathway
    Malcles, MH
    Cueille, N
    Mechali, F
    Coux, O
    Bonne-Andrea, C
    JOURNAL OF VIROLOGY, 2002, 76 (22) : 11350 - 11358