Development of biodegradable nanoparticles for delivery of quercetin

被引：356
作者：
Kumari, Avnesh
Yadav, Sudesh Kumar
Pakade, Yogesh B. [2 ]
Singh, Bikram [3 ]
Yadav, Subhash Chandra [1 ]
机构：
[1] CSIR, Inst Himalayan Bioresource Technol, Div Biotechnol, Nanobiol Lab, Palampur 176061, Himachal Prades, India
[2] CSIR, Inst Himalayan Bioresource Technol, Hill Area Tea Sci Div, Palampur 176061, Himachal Prades, India
[3] CSIR, Inst Himalayan Bioresource Technol, Nat Plant Prod Div, Palampur 176061, Himachal Prades, India
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2010年 / 80卷 / 02期
关键词：
Quercetin; PLA; Nanoparticles; Encapsulation efficiency; Antioxidant; In vitro release; BOVINE SERUM-ALBUMIN; ANTIOXIDANT ACTIVITY; IN-VITRO; FLUORESCENCE; DERIVATIVES; RELEASE; SYSTEMS;
D O I：
10.1016/j.colsurfb.2010.06.002
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
The antioxidant molecule quercetin has been encapsulated on poly-D,L-lactide (PLA) nanoparticles by solvent evaporation method for the improvement of its poor aqueous solubility and stability. The surface morphology and average size of PLA and quercetin loaded PLA nanoparticles are 170 +/- 25 and 130 +/- 30 nm respectively. The antioxidant activities of the PLA encapsulated quercetin nanomedicine are identical to free quercetin. The nanoencapsulation efficiency of quercetin evaluated by HPLC and antioxidant assay is 96.7%. The in vitro release kinetics under physiological condition show initial burst release followed by slow and sustained release. The complete release and maximum retention of quercetin is 72 and 96 h respectively. The less fluorescence quenching efficiency of quercetin-PLA nanoparticles than free quercetin on BSA confirms the controlled release of quercetin from PLA nanoparticles. These properties of PLA encapsulated quercetin molecule pave way for encapsulating various therapeutically less useful highly active antioxidant molecules towards the development of better therapeutic compounds. (C) 2010 Elsevier B.V. All rights reserved.
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收藏
页码：184 / 192
页数：9
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