Functional and biomechanical evaluation of a completely recellularized stentless pulmonary bioprosthesis in sheep

被引:10
作者
De Visscher, Geofrey [1 ]
Blockx, Helga [1 ]
Meuris, Bart [1 ]
Van Oosterwyck, Hans [2 ]
Verbeken, Erik [3 ]
Herregods, Marie-Christine [4 ]
Flameng, Willem [1 ]
机构
[1] Katholieke Univ Leuven, Dept Cardiovasc Dis, Lab Expt Cardiac Surg, B-3000 Louvain, Belgium
[2] Katholieke Univ Leuven, Div Biomech & Engn Design, Louvain, Belgium
[3] Univ Hosp Leuven, Dept Morphol & Mol Pathol, Louvain, Belgium
[4] Univ Hosp Leuven, Dept Cardiol, Louvain, Belgium
关键词
D O I
10.1016/j.jtcvs.2007.09.016
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: In a previous study we showed that recellularization of a stentless bioprosthetic valve is stimulated 1 month after implantation in the pulmonary position, when its matrix (acellular photo-oxidized bovine pericardium) was preseeded by intraperitoneal implantation during a 3-day period. Methods: The present study reports on the functional and biomechanical properties of such valves (n 5 19) in sheep up to 5 months after implantation. Similar valves (n 5 20) that were not intraperitoneally preseeded served as controls. Results: Recellularization was partial in control valves and excessive in preseeded valves: 66% versus 223% of cellularity of native valves, respectively (P < .05). The valves were endothelialized and contained interstitial cells depositing new matrix (collagens and elastin). However, phenotyping revealed an increased proportion of cells with contractile properties (30% -40% alpha smooth muscle actin 1) in both groups. Intraperitoneally seeded valves had thicker and shorter leaflets that were associated with mildly increased peak gradients and regurgitation. Characterization of the matrix properties revealed a gradually degrading matrix (625% loss of collagen organization at 5 months) and a concomitant alteration of its biomechanical properties, that is, decreased strength, stiffness, and maximum force. However, overall valve function remained intact, and the biomechanical properties of the whole valves were superior to that of the native valves. Conclusion: The ectopic in vivo seeding paradigm provides full recellularization. However, the volume fraction of the cellular phenotypes is not optimal, resulting in inadequate remodeling of the valves.
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收藏
页码:395 / 404
页数:10
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