PB@PDA@Ag nanosystem for synergistically eradicating MRSA and accelerating diabetic wound healing assisted with laser irradiation

被引:193
作者
Tong, Chunyi [1 ]
Zhong, Xianghua [2 ]
Yang, Yuejun [2 ]
Liu, Xu [3 ]
Zhong, Guowei [1 ]
Xiao, Chang [1 ]
Liu, Bin [1 ,4 ]
Wang, Wei [3 ]
Yang, Xiaoping [2 ]
机构
[1] Hunan Univ, Coll Biol, Changsha 410082, Peoples R China
[2] Hunan Normal Univ, Sch Med, Changsha 410125, Peoples R China
[3] Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Peoples R China
[4] Ningxia Med Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, NHC Key Lab Metab Cardiovasc Dis Res, Yinchuan 750004, Ningxia, Peoples R China
关键词
MRSA; PB@PDA@Ag nanocomposites; Photothermal effect; Diabetic wound; PHOTOTHERMAL ABLATION; SILVER NANOPARTICLES; ANTIBACTERIAL; TOXICITY; HYDROGEL; BACTERIA; PLATFORM; COPPER; NANO;
D O I
10.1016/j.biomaterials.2020.119936
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The ever-growing threats of multidrug-resistant bacterial infection and chronic wound healing have created an imperative need for the development of novel antibacterial materials and therapeutic strategies, especially for diabetic patients infected with multidrug-resistant bacteria. In this work, the nanocomplexes named as PB@ PDA@Ag were used for eradicating multidrug-resistant bacteria and accelerating wound healing of MRSA-infected diabetic model with the assistance of laser irradiation. In vitro results revealed that the combinational strategy exerted a synergistic effect for anti-MRSA through disrupting cell integrity, producing ROS, declining ATP, and oxidizing GSH, comparing with PB@PDA@Ag or NIR laser irradiation alone. Moreover, in vivo assay demonstrated that this system effectively accelerated MRSA-infected diabetic wound healing by mitigating local inflammatory response and up-regulating VEGF expression on the wound bed. Meanwhile, satisfactory biocompatibility and negligible damage to major organs were observed. Altogether, the aforementioned results indicate that the combinational therapy of PB@PDA@Ag and NIR irradiation shows a great potential application in the field of clinic infection.
引用
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页数:14
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