Three dimensional time-gated tracking of non-blinking quantum dots in live cells

被引：7
|
作者：
DeVore, Matthew S. [1 ]
Stich, Dominik G. [1 ]
Keller, Aaron M. [1 ]
Ghosh, Yagnaseni [1 ]
Goodwin, Peter M. [1 ]
Phipps, Mary E. [1 ]
Stewart, Michael H. [2 ]
Cleyrat, Cedric [3 ,4 ]
Wilson, Bridget S. [3 ,4 ]
Lidke, Diane S. [3 ,4 ]
Hollingsworth, Jennifer A. [1 ]
Werner, James H. [1 ]
机构：
[1] Los Alamos Natl Lab, Ctr Integrated Nanotechnol, Los Alamos, NM 87545 USA
[2] US Naval Res Lab, Div Opt Sci, Washington, DC 20375 USA
[3] Univ New Mexico, Dept Pathol, Albuquerque, NM 87131 USA
[4] Univ New Mexico, Canc Res & Treatment Ctr, Albuquerque, NM 87131 USA
来源：
COLLOIDAL NANOPARTICLES FOR BIOMEDICAL APPLICATIONS X | 2015年 / 9338卷
关键词：
Single particle tracking; time-gating; quantum dot; microscopy; SINGLE-PARTICLE TRACKING; SHELL-THICKNESS; PHOTOLUMINESCENCE; SUPPRESSION; PROTEINS; DYNAMICS;
D O I：
10.1117/12.2082943
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. Signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.
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页数：15
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