Sonic Hedgehog Signaling Mediates Resveratrol to Increase Proliferation of Neural Stem Cells After Oxygen-Glucose Deprivation/Reoxygenation Injury in Vitro

被引：48

作者：

Cheng, Wei ^{[1
]}

Yu, Pingping ^{[1
]}

Wang, Li ^{[1
]}

Shen, Changbo ^{[1
]}

Song, Xiaosong ^{[1
]}

Chen, Jixiang ^{[1
]}

Tang, Fanren ^{[1
]}

Yang, Qin ^{[1
]}

机构：

[1] Chongqing Med Univ, Affiliated Hosp 1, Dept Neurol, Chongqing 400016, Peoples R China

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2015年 / 35卷 / 05期

关键词：

Reservatrol; Neural stem cells; Sonic hedgehog signaling; Proliferation; Cyclopamine; FOCAL CEREBRAL-ISCHEMIA; HIPPOCAMPAL NEUROGENESIS; CORTICAL-NEURONS; PRECURSOR CELLS; BRAIN; DEPRIVATION; STROKE; DIFFERENTIATION; MECHANISMS; APOPTOSIS;

D O I：

10.1159/000374009

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background/Aims: There is interest in drugs and rehabilitation methods to enhance neurogenesis and improve neurological function after brain injury or degeneration. Resveratrol may enhance hippocampal neurogenesis and improve hippocampal atrophy in chronic fatigue mice and prenatally stressed rats. However, its effect and mechanism of neurogenesis after stroke is less well understood. Sonic hedgehog (Shh) signaling is crucial for neurogenesis in the embryonic and adult brain, but relatively little is known about the role of Shh signaling in resveratrol-enhanced neurogenesis after stroke. Methods: Neural stem cells (NSCs) before oxygen-glucose deprivation/reoxygenation (OGD/R) in vitro were pretreated with resveratrol with or without cyclopamine. Survival and proliferation of NSCs was assessed by the CCK8 assay and BrdU immunocytochemical staining. The expressions and activity of signaling proteins and mRNAs were detected by immunocytochemistry, Western blotting, and RT-PCR analysis. Results: Resveratrol significantly increased NSCs survival and proliferation in a concentration-dependent manner after OGD/R injury in vitro. At the same time, the expression of Patched-1, Smoothened (Smo), and Gli-1 proteins and mRNAs was upregulated, and Gli-1 entered the nucleus, which was inhibited by cyclopamine, a Smo inhibitor. Conclusion: Shh signaling mediates resveratrol to increase NSCs proliferation after OGD/R injury in vitro. Copyright (C) 2015 S. Karger AG, Basel

引用

页码：2019 / 2032

页数：14

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