Microvessels support engraftment and functionality of human islets and hESC-derived pancreatic progenitors in diabetes models

被引：69

作者：

Aghazadeh, Yasaman ^{[1
,2
]}

Poon, Frankie ^{[1
,3
]}

Sarangi, Farida ^{[1
]}

Wong, Frances T. M. ^{[3
]}

Khan, Safwat T. ^{[2
,4
]}

Sun, Xuetao ^{[2
]}

Hatkar, Rupal ^{[2
]}

Cox, Brian J. ^{[3
,5
]}

Nunes, Sara S. ^{[2
,4
,6
,7
]}

Nostro, M. Cristina ^{[1
,3
]}

机构：

[1] Univ Hlth Network, McEwen Stem Cell Inst, Toronto, ON M5G 1L7, Canada

[2] Univ Hlth Network, Toronto Gen Hosp Res Inst, Toronto, ON M5G IL7, Canada

[3] Univ Toronto, Dept Physiol, Toronto, ON M5S 1A8, Canada

[4] Univ Toronto, Inst Biomed Engn, Toronto, ON M5S 3G9, Canada

[5] Univ Toronto, Dept Obstet & Gynecol, Toronto, ON M5G 1E2, Canada

[6] Univ Toronto, Lab Med & Pathobiol, Toronto, ON M5S 1A8, Canada

[7] Univ Toronto, Heart & Stroke Richard Lewar Ctr Excellence, Toronto, ON M5S 3H2, Canada

来源：

CELL STEM CELL | 2021年 / 28卷 / 11期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

GROWTH-FACTOR; TRANSCRIPTION FACTOR; ENDOTHELIAL-CELLS; BETA-CELLS; IN-VITRO; STEM; VASCULARIZATION; FRAGMENTS; TISSUE; DIFFERENTIATION;

D O I：

10.1016/j.stem.2021.08.001

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Islet transplantation is a promising treatment for type 1 diabetes (T1D), yet the low donor pool, poor islet engraftment, and life-long immunosuppression prevent it from becoming the standard of care. Human embryonic stem cell (hESC)-derived pancreatic cells could eliminate donor shortages, but interventions to improve graft survival are needed. Here, we enhanced subcutaneous engraftment by employing a unique vascularization strategy based on ready-made microvessels (MVs) isolated from the adipose tissue. This resulted in improved cell survival and effective glucose response of both human islets and hESC-derived pancreatic cells, which ameliorated preexisting diabetes in three mouse models of T1D.

引用

页码：1936 / +

页数：23

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