Voltammetric behavior of the alizarin red S interaction with DNA and damage to DNA

被引：13

作者：

Yang, Zhou-Sheng ^{[1
]}

Zhang, Da-Peng ^{[1
]}

Long, Hai-Yan ^{[1
]}

Zhao, Guang-Chao ^{[1
]}

机构：

[1] Anhui Normal Univ, Coll Chem & Mat Sci, Anhui Key Lab Chemobiosensing, Wuhu 241000, Peoples R China

来源：

ELECTROANALYSIS | 2007年 / 19卷 / 24期

关键词：

alizarin red S; DNA modified electrode; interaction; DNA damage;

D O I：

10.1002/elan.200704016

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The electrochemical behavior and the interaction of alizarin red S (ARS) with calf thymus DNA was investigated on a bare glassy carbon electrode (GCE) and DNA modified GCE (DNA/GCE), respectively. ARS showed a pair of redox peaks at -0.445 V and -0.414 V on a bare GCE. On addition of DNA into the ARS solution, the peak current of ARS decreased and the peak potential positively shifted, but without new redox peaks appeared. The ARS reduction peak current increased with immersion time on a DNA/GCE. The results showed that ARS could interact with DNA molecules by intercalative binding mode. The equilibrium constant, binding number and the ratio of binding constant for oxidized and reduced ARS forms were obtained. The DNA damage was directly detected by appearance of guanosine and adenosine bases oxidation signal. The influence of experimental conditions on DNA damage extent was discussed in detail.

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收藏

页码：2577 / 2582

页数：6

相关论文

共 39 条

[1] INTERACTION BETWEEN ACRIDINE DYES AND DEOXYRIBONUCLEIC ACID [J].

ARMSTRONG, RW ;

STRAUSS, UP ;

KURUCSEV, T .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1970, 92 (10) :3174-+

[2] Anti-human cytomegalovirus activity and toxicity of sulfonated anthraquinones and anthraquinone derivatives [J].

Barnard, DL ;

Fairbairn, DW ;

ONeill, KL ;

Gage, TL ;

Sidwell, RW .

ANTIVIRAL RESEARCH, 1995, 28 (04) :317-329

[3] An electrical probe of protein-DNA interactions on DNA-modified surfaces [J].

Boon, EM ;

Salas, JE ;

Barton, JK .

NATURE BIOTECHNOLOGY, 2002, 20 (03) :282-286

[4] Voltammetric behaviour of mitoxantrone at a DNA-biosensor [J].

Brett, AMO ;

Macedo, TRA ;

Raimundo, D ;

Marques, MH ;

Serrano, SHP .

BIOSENSORS & BIOELECTRONICS, 1998, 13 (7-8) :861-867

[5] Detection of damage to DNA and antioxidative activity of yeast polysaccharides at the DNA-modified screen-printed electrode [J].

Buková, M ;

Labuda, J ;

Sandula, J ;

Krizková, L ;

Stepánek, I ;

Duracková, Z .

TALANTA, 2002, 56 (05) :939-947

[6] VOLTAMMETRIC STUDIES OF THE INTERACTION OF METAL-CHELATES WITH DNA .2. TRIS-CHELATED COMPLEXES OF COBALT(III) AND IRON(II) WITH 1,10-PHENANTHROLINE AND 2,2'-BIPYRIDINE [J].

CARTER, MT ;

RODRIGUEZ, M ;

BARD, AJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (24) :8901-8911

[7] Voltammetric behavior of alizarin red S adsorbed on electrochemically pretreated glassy carbon electrodes [J].

Dai, HP ;

Shiu, KK .

ELECTROCHIMICA ACTA, 1998, 43 (18) :2709-2715

[8] Electrochemistry of nucleic acids at solid electrodes and its applications [J].

de-los-Santos-Alvarez, P ;

Lobo-Castañón, MJ ;

Miranda-Ordieres, AJ ;

Tuñón-Blanco, P .

ELECTROANALYSIS, 2004, 16 (15) :1193-1204

[9] Electrochemical degradation of anthraquinone dye Alizarin Red S by anodic oxidation on boron-doped diamond [J].

Faouzi, Ahmadi Mohamed ;

Nasr, Bensalah ;

Abdellatif, Gadri .

DYES AND PIGMENTS, 2007, 73 (01) :86-89

[10] Electrochemical studies of porphyrin interacting with DNA and determination of DNA [J].

Feng, Q ;

Li, NQ ;

Jiang, YY .

ANALYTICA CHIMICA ACTA, 1997, 344 (1-2) :97-104

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