A high-throughput screening method for endophytic bacteria with antagonistic activity against Magnaporthe oryzae in rice (Oryza sativa L.) seeds

被引：2

作者：

Wang, Zhishan ^{[1
]}

Wu, Xianyu ^{[1
]}

Li, Ni ^{[2
]}

Wang, Weiping ^{[2
]}

Liu, Yang ^{[1
]}

机构：

[1] Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Beijing 100083, Peoples R China

[2] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China

来源：

PLANT GROWTH REGULATION | 2022年 / 96卷 / 02期

关键词：

Rice seed; Endophytes; High-throughput screening; Antifungal activity; Biological control; ZEA-MAYS L; COMMUNITY; DIVERSITY; STRAIN;

D O I：

10.1007/s10725-021-00771-z

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Screening for microorganisms with antagonistic activity against crop pathogens is essential for microbial antagonist preparation, crop disease control, and sustainable agricultural development. This study established a rapid, efficient, one-step high-throughput method for screening endophytic bacteria displaying antagonistic activity against Magnaporthe oryzae (M. oryzae) ACCC36020 in rice seeds. The experimental results indicated that these bacteria could be directly screened from six sample groups using this method. This technique can achieve simultaneous screening and purification, identify endophytic bacteria displaying antifungal activity in rice seeds, and save significant time.

引用

页码：237 / 241

页数：5

共 28 条

[1] A high-throughput screening method for endophytic bacteria with antagonistic activity against Magnaporthe oryzae in rice (Oryza sativa L.) seeds
Zhishan Wang
Xianyu Wu
Ni Li
Weiping Wang
Yang Liu
Plant Growth Regulation, 2022, 96 : 237 - 241
[2] Genetic characterization of rice endophytic bacteria (Oryza sativa L.) with antimicrobial activity against Burkholderia glumae
Valdez-Nunez, Renzo A.
Rios-Ruiz, Winston F.
Ormeno-Orrillo, Ernesto
Torres-Chavez, Edson E.
Torres-Delgado, Jorge
REVISTA ARGENTINA DE MICROBIOLOGIA, 2020, 52 (04): : 315 - 327
[3] An endophytic strain JK of genus bacillus isolated from the seeds of super hybrid rice (Oryza sativa L., Shenliangyou 5814) has antagonistic activity against rice blast pathogen
Jing, Ruixue
Li, Ni
Wang, Weiping
Liu, Yang
MICROBIAL PATHOGENESIS, 2020, 147
[4] Study on the diversity of endophytic communities from rice (Oryza sativa L.) and their antagonistic activities in vitro
Naik, B. Shankar
Shashikala, J.
Krishnamurthy, Y. L.
MICROBIOLOGICAL RESEARCH, 2009, 164 (03) : 290 - 296
[5] Identification of endophytic bacterial strain RSE1 from seeds of super hybrid rice Shenliangyou 5814 (Oryza sativa L.,) and evaluation of its antagonistic activity
Liu, Yang
Bai, Feirong
Li, Ni
Wang, Weiping
Cheng, Chi
PLANT GROWTH REGULATION, 2017, 82 (03) : 403 - 408
[6] Identification of endophytic bacterial strain RSE1 from seeds of super hybrid rice Shenliangyou 5814 (Oryza sativa L.,) and evaluation of its antagonistic activity
Yang Liu
Feirong Bai
Ni Li
Weiping Wang
Chi Cheng
Plant Growth Regulation, 2017, 82 : 403 - 408
[7] Insights into Endophytic Bacterial Community Structures of Seeds Among Various Oryza sativa L. Rice Genotypes
Zhang, Jun
Zhang, Caiwen
Yang, Jing
Zhang, Ruijie
Gao, Jusheng
Zhao, Xia
Zhao, Juanjuan
Zhao, Dongfang
Zhang, Xiaoxia
JOURNAL OF PLANT GROWTH REGULATION, 2019, 38 (01) : 93 - 102
[8] Endophytic Seed-Associated Bacteria as Plant Growth Promoters of Cuban Rice (Oryza sativa L.)
Hernandez, Ionel
Taule, Cecilia
Perez-Perez, Renee
Battistoni, Federico
Fabiano, Elena
Villanueva-Guerrero, Angela
Napoles, Maria Caridad
Herrera, Hector
MICROORGANISMS, 2023, 11 (09)
[9] Antimicrobial activity of essential oils against seed borne fungi of rice (Oryza sativa L.)
Muthukumar, A.
Sangeetha, G.
Naveenkumar, R.
JOURNAL OF ENVIRONMENTAL BIOLOGY, 2016, 37 (06) : 1429 - 1436
[10] Plant Growth-Promoting Activity of Bacteria Isolated from Asian Rice (Oryza sativa L.) Depends on Rice Genotype
Balasjin, Nasim Maghboli
Maki, James S.
Schlappi, Michael R.
Marshall, Christopher W.
MICROBIOLOGY SPECTRUM, 2022, 10 (04):

← 1 2 3 →