A streamlined protocol for emulsion polymerase chain reaction and subsequent purification

被引：48

作者：

Schuetze, Tatjana ^{[1
,2
]}

Rubelt, Florian ^{[1
,2
]}

Repkow, Julia ^{[1
]}

Greiner, Nicole ^{[1
]}

Erdmann, Volker A. ^{[2
]}

Lehrach, Hans ^{[1
]}

Konthur, Zoltan ^{[1
]}

Gloekler, Joern ^{[1
]}

机构：

[1] Max Planck Inst Mol Genet, Dept Vertebrate Genom, D-14195 Berlin, Germany

[2] Free Univ Berlin, Inst Chem Biochem, D-14195 Berlin, Germany

来源：

ANALYTICAL BIOCHEMISTRY | 2011年 / 410卷 / 01期

关键词：

PCR; DNA;

D O I：

10.1016/j.ab.2010.11.029

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Compartmentalization of polymerase chain reaction (PCR) reduces artifacts, especially when complex libraries are amplified. It allows clonal amplification of templates from complex mixtures in a bias-free manner. Here we describe a rapid, straightforward, and easy protocol for PCR in a water-in-oil emulsion (ePCR) including sample recovery by DNA purification. Furthermore, no special laboratory equipment is needed and inexpensive components are used. Therefore, our flexible protocol allows ePCR to be readily implemented in daily routine experiments for a broad range of applications. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：155 / 157

页数：3

共 9 条

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