The administration of argon during ex vivo normothermic perfusion in an experimental model of kidney ischemia-reperfusion injury

被引:18
作者
Smith, Stephanie F. [1 ]
Adams, Thomas [1 ]
Hosgood, Sarah A. [1 ]
Nicholson, Michael L. [1 ]
机构
[1] Univ Cambridge, Addenbrookes Hosp, Dept Surg, Box 202,Level 9,Hills Rd, Cambridge CB2 0QQ, England
关键词
Ischemia-reperfusion injury; EVNP; Argon; Kidney; HIF-1; alpha; RENAL PRESERVATION; NOBLE-GASES;
D O I
10.1016/j.jss.2017.05.041
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background: Argon has shown potential as an organoprotective agent in numerous models of ischemia-reperfusion injury (IRI). The aim of this study was to evaluate the effects of argon gas during ex vivo normothermic perfusion (EVNP) in an experimental porcine model of kidney IRI. Materials and methods: After a warm ischemia time of 15 min and 17 h of static cold storage, porcine kidneys underwent 1 h of EVNP using leukocyte-depleted blood. During EVNP, kidneys were perfused with a gas composition either of 70% argon (n = 6), 70% nitrogen control (n = 6), or standard 95% oxygen (n = 6) balanced with 5% carbon dioxide. After EVNP, kidneys were reperfused with whole blood under standard conditions for 3 h to assess renal function and injury. Results: During 1-h EVNP, the mean renal blood flow was numerically higher in the argon group (49.2 +/- 16.2 mL/min/100 g; P = 0.320) compared with the nitrogen and oxygen groups (42.9 +/- 18.64 and 37.71 +/- 7.0 mL/min/100 g, respectively). Other measures of renal function and hemodynamics were not significantly different between the argon and control groups during this period. During reperfusion, no significant differences were found in functional parameters or inflammatory markers (P < 0.05). Histologic analysis revealed no significant change in morphology or hypoxia-inducible factor-1 alpha staining between gaseous groups. Nuclear hypoxia-inducible factor-1 alpha staining was observed only after 3 h of reperfusion. Conclusions: Our findings suggest that using 70% argon during 1 h of EVNP does not mediate a measurable organoprotective effect in an experimental porcine model of IRI. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码:202 / 208
页数:7
相关论文
共 30 条
  • [1] Experimental renal preservation by normothermic resuscitation perfusion with autologous blood
    Bagul, A.
    Hosgood, S. A.
    Kaushik, M.
    Kay, M. D.
    Waller, H. L.
    Nicholson, M. L.
    [J]. BRITISH JOURNAL OF SURGERY, 2008, 95 (01) : 111 - 118
  • [2] Transport of hypoxia-inducible factor HIF-1α into the nucleus involves importins 4 and 7
    Chachami, Georgia
    Paraskeva, Efrosyni
    Mingot, Jose-Manuel
    Braliou, Georgia G.
    Goerlich, Dirk
    Simos, George
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2009, 390 (02) : 235 - 240
  • [3] Hypoxia Inducible Factor 1-Alpha (HIF-1 Alpha) Is Induced during Reperfusion after Renal Ischemia and Is Critical for Proximal Tubule Cell Survival
    Conde, Elisa
    Alegre, Laura
    Blanco-Sanchez, Ignacio
    Saenz-Morales, David
    Aguado-Fraile, Elia
    Ponte, Belen
    Ramos, Edurne
    Saiz, Ana
    Jimenez, Carlos
    Ordonez, Angel
    Lopez-Cabrera, Manuel
    del Peso, Luis
    de Landazuri, Manuel O.
    Liano, Fernando
    Selgas, Rafael
    Antonio Sanchez-Tomero, Jose
    Laura Garcia-Bermejo, Maria
    [J]. PLOS ONE, 2012, 7 (03):
  • [4] The Efficacy of Noble Gases in the Attenuation of Ischemia Reperfusion Injury: A Systematic Review and Meta-Analyses
    De Deken, Julie
    Rex, Steffen
    Monbaliu, Diethard
    Pirenne, Jacques
    Jochmans, Ina
    [J]. CRITICAL CARE MEDICINE, 2016, 44 (09) : E886 - E896
  • [5] Effectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantation
    Faure, Alice
    Bruzzese, Laurie
    Steinberg, Jean-Guillaume
    Jammes, Yves
    Torrents, Julia
    Berdah, Stephane V.
    Garnier, Emmanuelle
    Legris, Tristan
    Loundou, Anderson
    Chalopin, Matthieu
    Magalon, Guy
    Guieu, Regis
    Fenouillet, Emmanuel
    Lechevallier, Eric
    [J]. JOURNAL OF TRANSLATIONAL MEDICINE, 2016, 14
  • [6] Metabolic shift in liver: Correlation between perfusion temperature and hypoxia inducible factor-1α
    Ferrigno, Andrea
    Di Pasqua, Laura Giuseppina
    Bianchi, Alberto
    Richelmi, Plinio
    Vairetti, Mariapia
    [J]. WORLD JOURNAL OF GASTROENTEROLOGY, 2015, 21 (04) : 1108 - 1116
  • [7] Oxygen-dependent ubiquitination and degradation of hypoxia-inducible factor requires nuclear-cytoplasmic trafficking of the von Hippel-Lindau tumor suppressor protein
    Groulx, I
    Lee, S
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2002, 22 (15) : 5319 - 5336
  • [8] Leucocyte depletion improves renal function during reperfusion using an experimental isolated haemoperfused organ preservation system
    Harper, S.
    Hosgood, S.
    Kay, M.
    Nicholson, M.
    [J]. BRITISH JOURNAL OF SURGERY, 2006, 93 (05) : 623 - 629
  • [9] Effects of arterial pressure in an experimental isolated haemoperfused porcine kidney preservation system
    Hosgood, S.
    Harper, S.
    Kay, M.
    Bagul, A.
    Waller, H.
    Nicholson, M. L.
    [J]. BRITISH JOURNAL OF SURGERY, 2006, 93 (07) : 879 - 884
  • [10] Successful Transplantation of Human Kidneys Deemed Untransplantable but Resuscitated by Ex Vivo Normothermic Machine Perfusion
    Hosgood, S. A.
    Saeb-Parsy, K.
    Hamed, M. O.
    Nicholson, M. L.
    [J]. AMERICAN JOURNAL OF TRANSPLANTATION, 2016, 16 (11) : 3282 - 3285