Rapid and validated HPLC assay for the determination of oxytetracycline in biological material

A rapid, accurate, simple, and reproducible high performance liquid chromatographic (HPLC) method for the determination of oxytetracycline hydrochloride (OTC) in porcine plasma has been developed and validated. The drug and the standard were eluted from 5 gm Omni Spher (Varian) C18 column (250 mm x 4.6 mm I.D.) at room temperature. The mobile phase was composed of ACN-MeOH-(HCOO)(2) (17.5:17.5:65 v/v/v) (pH adjusted to 2). A flow rate was 1.4 mL/min. The effluent was monitored using a UV-VIS detector set at 360 nm. The retention time of OTC was about 3 min. The suggested technique is characterised by superior performance parameters: linearity R-2 = 0.9999, recovery = 92.50%, repeatability RSD <= 1.39%. These results demonstrate the validity of the HPLC method for the analysis of OTC HCL.