The influence of sex and estrous cycle on QTL for emotionality and ethanol consumption

被引:16
作者
Izidio, Geison S. [1 ,2 ]
Oliveira, Leticia C. [2 ]
Oliveira, Ligia F. G. [2 ]
Pereira, Elayne [2 ]
Wehrmeister, Thaize D. [2 ]
Ramos, Andre [2 ]
机构
[1] Univ Fed Rio Grande do Norte, Lab Estudos Memoria Roedores, Dept Fisiol, Ctr Biociencias, BR-59078970 Natal, RN, Brazil
[2] Univ Fed Santa Catarina, Lab Genet Comportamento, Dept Biol Celular Embriol & Genet, BR-88040900 Florianopolis, SC, Brazil
关键词
QUANTITATIVE TRAIT LOCUS; ANXIETY-RELATED BEHAVIORS; OPEN-FIELD; ANIMAL-MODELS; ALCOHOL-CONSUMPTION; OVARIAN HORMONES; ALPHA-SYNUCLEIN; BLOOD-PRESSURE; SOCIAL STRESS; GENETIC-BASIS;
D O I
10.1007/s00335-011-9327-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The inbred rat strains Lewis (LEW) and Spontaneously Hypertensive Rats (SHR) differ with respect to several emotionality- and ethanol intake-related behaviors, one of which (inner locomotion in the open field; OF) is strongly influenced by a locus (Anxrr16) on chromosome (Chr) 4. We aimed to further investigate the influence of Chr 4 on these behaviors and to evaluate the role of the estrous cycle in QTL expression. LEW females and SHR males were intercrossed to produce F1 and F2 rats (96-97/sex), which were then tested in the OF, light-dark box (LDB), forced swimming test (FST), and an ethanol consumption procedure (ECP). In addition, another group of 96 F2 females were tested in the OF and LDB according to their estrous cycle phase. All animals were genotyped for microsatellite markers located on Chr 4 and two QTL analyses were performed. A factor analysis of the F2 population produced five factors reflecting different behavioral dimensions. QTL analysis revealed five significant loci in males, some of which with pleiotropic effects on behaviors measured in the OF, LDB, and ECP. The second QTL analysis revealed two significant loci in females in diestrous-proestrous and one in females in estrous-metestrous that influence behaviors in the OF and LDB. Results revealed that Anxrr16 and four other new QTL influence emotionality- and ethanol-related behaviors in male rats, whereas Anxrr16 attained suggestive levels only in females in diestrous-proestrous, which raises the need for taking into account factors related to the sex and estrous cycle in behavioral QTL analysis.
引用
收藏
页码:329 / 340
页数:12
相关论文
共 86 条
[1]   Effects of propranolol, buspirone, pCPA, reserpine, and chlordiazepoxide on open-field behavior [J].
Angrini, M ;
Leslie, JC ;
Shephard, RA .
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 1998, 59 (02) :387-397
[2]   TESTS FOR EMOTIONALITY IN RATS AND MICE - REVIEW [J].
ARCHER, J .
ANIMAL BEHAVIOUR, 1973, 21 (MAY) :205-235
[3]   The alcohol-preferring P rat and animal models of excessive alcohol drinking [J].
Bell, Richard L. ;
Rodd, Zachary A. ;
Lumeng, Lawrence ;
Murphy, James M. ;
McBride, William J. .
ADDICTION BIOLOGY, 2006, 11 (3-4) :270-288
[4]   Daily patterns of ethanol drinking in peri-adolescent and adult alcohol-preferring (P) rats [J].
Bell, RL ;
Rodd, ZA ;
Sable, HJK ;
Schultz, JA ;
Hsu, CC ;
Lumeng, L ;
Murphy, JM ;
McBride, WJ .
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 2006, 83 (01) :35-46
[5]  
Berton O, 1998, NEUROSCIENCE, V82, P147
[6]   Genomic screen for QTLs underlying alcohol consumption in the P and NP rat lines [J].
Bice, P ;
Foroud, T ;
Bo, RH ;
Castelluccio, P ;
Lumeng, L ;
Li, TK ;
Carr, LG .
MAMMALIAN GENOME, 1998, 9 (12) :949-955
[7]  
Cailhol S, 2002, J STUD ALCOHOL, V63, P91
[8]   Quantitative trait loci analysis affecting contextual conditioning in mice [J].
Caldarone, B ;
Saavedra, C ;
Tartaglia, K ;
Wehner, JM ;
Dudek, BC ;
Flaherty, L .
NATURE GENETICS, 1997, 17 (03) :335-337
[9]   A quantitative trait locus for alcohol consumption in selectively bred rat lines [J].
Carr, LG ;
Foroud, T ;
Bice, P ;
Gobbett, T ;
Ivashina, J ;
Edenberg, H ;
Lumeng, L ;
Li, TK .
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 1998, 22 (04) :884-887
[10]   Influence of life stress on depression: Moderation by a polymorphism in the 5-HTT gene [J].
Caspi, A ;
Sugden, K ;
Moffitt, TE ;
Taylor, A ;
Craig, IW ;
Harrington, H ;
McClay, J ;
Mill, J ;
Martin, J ;
Braithwaite, A ;
Poulton, R .
SCIENCE, 2003, 301 (5631) :386-389