Simplified process for ethanol production from sugarcane bagasse using hydrolysate-resistant Escherichia coli strain MM160

被引：85

作者：

Geddes, C. C. ^{[1
]}

Mullinnix, M. T. ^{[1
]}

Nieves, I. U. ^{[1
]}

Peterson, J. J. ^{[1
]}

Hoffman, R. W. ^{[2
]}

York, S. W. ^{[1
]}

Yomano, L. P. ^{[1
]}

Miller, E. N. ^{[1
]}

Shanmugam, K. T. ^{[1
]}

Ingram, L. O. ^{[1
]}

机构：

[1] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA

[2] Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA

来源：

BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 03期

关键词：

Cellulase; Lignocellulose; SSF; L plus SScF; Ethanol; SIMULTANEOUS SACCHARIFICATION; CORN STOVER; AQUEOUS AMMONIA; PRETREATMENT; COFERMENTATION; XYLOSE; IDENTIFICATION; FERMENTATION; INHIBITORS; SOAKING;

D O I：

10.1016/j.biortech.2010.10.143

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Hexose and pentose sugars from phosphoric acid pretreated sugarcane bagasse were co-fermented to ethanol in a single vessel (SScF), eliminating process steps for solid-liquid separation and sugar cleanup. An initial liquefaction step (L) with cellulase was included to improve mixing and saccharification (L + SScF), analogous to a corn ethanol process. Fermentation was enabled by the development of a hydrolysate-resistant mutant of Escherichia coli LY180, designated MM160. Strain MM160 was more resistant than the parent to inhibitors (furfural, 5-hydroxymethylfurfural, and acetate) formed during pretreatment. Bagasse slurries containing 10% and 14% dry weight (fiber plus solubles) were tested using pretreatment temperatures of 160-190 degrees C (1% phosphoric acid, 10 min). Enzymatic saccharification and inhibitor production both increased with pretreatment temperature. The highest titer (30 g/L ethanol) and yield (0.21 g ethanol/g bagasse dry weight) were obtained after incubation for 122 h using 14% dry weight slurries of pretreated bagasse (180 degrees C). (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：2702 / 2711

页数：10

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