Analysis of PRODUCTION OF FLAVONOL GLYCOSIDES-dependent flavonol glycoside accumulation in Arabidopsis thaliana plants reveals MYB11-, MYB12-and MYB111-independent flavonol glycoside accumulation

被引：271

作者：

Stracke, Ralf ^{[1
]}

Jahns, Oliver ^{[1
]}

Keck, Matthias ^{[1
]}

Tohge, Takayuki ^{[2
,3
]}

Niehaus, Karsten ^{[1
]}

Fernie, Alisdair R. ^{[3
]}

Weisshaar, Bernd ^{[1
]}

机构：

[1] Univ Bielefeld, Dept Biol, D-33594 Bielefeld, Germany

[2] RIKEN Plant Sci Ctr, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

[3] Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany

来源：

NEW PHYTOLOGIST | 2010年 / 188卷 / 04期

关键词：

Arabidopsis thaliana; flavonoid biosynthesis; flavonol glycosides; flavonols; gene regulation; R2R3-MYB; UV-B RADIATION; AUXIN TRANSPORT; PHENYLPROPANOID BIOSYNTHESIS; DOMAIN PROTEIN; GENE ENCODES; EXPRESSION; R2R3-MYB; MUTANTS; BIOCHEMISTRY; GROWTH;

D O I：

10.1111/j.1469-8137.2010.03421.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

P>The flavonol branch of flavonoid biosynthesis is under transcriptional control of the R2R3-MYBs PRODUCTION OF FLAVONOL GLYCOSIDE1 (PFG1/MYB12, PFG2/MYB11 and PFG3/MYB111) in Arabidopsis thaliana. Here, we investigated the influence of specific PFG transcription factors on flavonol distribution in various organs. A combination of genetic and metabolite analysis was used to identify transcription factor gene-metabolite correlations of the flavonol metabolic pathway. Flavonol glycoside accumulation patterns have been analysed in wild-type and multiple R2R3-MYB PFG mutants in an organ- and development-dependent manner using high-performance thin-layer chromatography, supplemented with liquid chromatography-mass spectroscopy metabolite profiling. Our results clearly demonstrate a differential influence of MYB11, MYB12 and MYB111 on the spatial accumulation of specific flavonol derivatives in leaves, stems, inflorescences, siliques and roots. In addition, MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation was observed in pollen grains and siliques/seeds. The highly complex tissue- and developmental-specific regulation of flavonol biosynthesis in A. thaliana is orchestrated by at least four PFG transcription factors, differentially influencing the spatial accumulation of specific flavonol derivatives. We present evidence that a separate flavonol control mechanism might be at play in pollen.

引用

页码：985 / 1000

页数：16

共 76 条

[31] BIOCHEMICAL COMPLEMENTATION OF CHALCONE SYNTHASE MUTANTS DEFINES A ROLE FOR FLAVONOLS IN FUNCTIONAL POLLEN [J].

MO, YY ;

NAGEL, C ;

TAYLOR, LP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (15) :7213-7217

[32]

Mol J, 1996, CRIT REV PLANT SCI, V15, P525, DOI 10.1080/07352689609382369

[33] Regulation of auxin transport by aminopeptidases and endogenous flavonoids [J].

Murphy, A ;

Peer, WA ;

Taiz, L .

PLANTA, 2000, 211 (03) :315-324

[34] Metabolomics-oriented isolation and structure elucidation of 37 compounds including two anthocyanins from Arabidopsis thaliana [J].

Nakabayashi, Ryo ;

Kusano, Miyako ;

Kobayashi, Makoto ;

Tohge, Takayuki ;

Yonekura-Sakakibara, Keiko ;

Kogure, Noriyuki ;

Yamazaki, Mami ;

Kitajima, Mariko ;

Saito, Kazuki ;

Takayama, Hiromitsu .

PHYTOCHEMISTRY, 2009, 70 (08) :1017-1029

[35] The TT8 gene encodes a basic helix-loop-helix domain protein required for expression of DFR and BAN genes in Arabidopsis siliques [J].

Nesi, N ;

Debeaujon, I ;

Jond, C ;

Pelletier, G ;

Caboche, M ;

Lepiniec, L .

PLANT CELL, 2000, 12 (10) :1863-1878

[36]

Nesi N, 2001, PLANT CELL, V13, P2099, DOI 10.1105/TPC.010098

[37] CHELATE VON DIARYLBORSAUREN MIT ALIPHATISCHEN OXYALKYLAMINEN ALS REAGENZIEN FUR DEN NACHWEIS VON OXYPHENYL-BENZO-GAMMA-PYRONEN [J].

NEU, R .

NATURWISSENSCHAFTEN, 1957, 44 (06) :181-182

[38] Differential flavonoid response to enhanced UV-B radiation in Brassica napus [J].

Olsson, LC ;

Veit, M ;

Weissenböck, G ;

Bornman, JF .

PHYTOCHEMISTRY, 1998, 49 (04) :1021-1028

[39]

ORMROD DP, 1995, PHYSIOL PLANTARUM, V93, P602, DOI 10.1111/j.1399-3054.1995.tb05106.x

[40] Biochemical and genetic characterization of Arabidopsis flavanone 3β-hydroxylase [J].

Owens, Daniel K. ;

Crosby, Kevin C. ;

Runac, Justin ;

Howard, Brad A. ;

Winkel, Brenda S. J. .

PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2008, 46 (10) :833-843

← 1 2 3 4 5 6 7 8 →