Analysis of PRODUCTION OF FLAVONOL GLYCOSIDES-dependent flavonol glycoside accumulation in Arabidopsis thaliana plants reveals MYB11-, MYB12-and MYB111-independent flavonol glycoside accumulation

被引：260
作者：
Stracke, Ralf [1 ]
Jahns, Oliver [1 ]
Keck, Matthias [1 ]
Tohge, Takayuki [2 ,3 ]
Niehaus, Karsten [1 ]
Fernie, Alisdair R. [3 ]
Weisshaar, Bernd [1 ]
机构：
[1] Univ Bielefeld, Dept Biol, D-33594 Bielefeld, Germany
[2] RIKEN Plant Sci Ctr, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan
[3] Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany
来源：
NEW PHYTOLOGIST | 2010年 / 188卷 / 04期
关键词：
Arabidopsis thaliana; flavonoid biosynthesis; flavonol glycosides; flavonols; gene regulation; R2R3-MYB; UV-B RADIATION; AUXIN TRANSPORT; PHENYLPROPANOID BIOSYNTHESIS; DOMAIN PROTEIN; GENE ENCODES; EXPRESSION; R2R3-MYB; MUTANTS; BIOCHEMISTRY; GROWTH;
D O I：
10.1111/j.1469-8137.2010.03421.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
P>The flavonol branch of flavonoid biosynthesis is under transcriptional control of the R2R3-MYBs PRODUCTION OF FLAVONOL GLYCOSIDE1 (PFG1/MYB12, PFG2/MYB11 and PFG3/MYB111) in Arabidopsis thaliana. Here, we investigated the influence of specific PFG transcription factors on flavonol distribution in various organs. A combination of genetic and metabolite analysis was used to identify transcription factor gene-metabolite correlations of the flavonol metabolic pathway. Flavonol glycoside accumulation patterns have been analysed in wild-type and multiple R2R3-MYB PFG mutants in an organ- and development-dependent manner using high-performance thin-layer chromatography, supplemented with liquid chromatography-mass spectroscopy metabolite profiling. Our results clearly demonstrate a differential influence of MYB11, MYB12 and MYB111 on the spatial accumulation of specific flavonol derivatives in leaves, stems, inflorescences, siliques and roots. In addition, MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation was observed in pollen grains and siliques/seeds. The highly complex tissue- and developmental-specific regulation of flavonol biosynthesis in A. thaliana is orchestrated by at least four PFG transcription factors, differentially influencing the spatial accumulation of specific flavonol derivatives. We present evidence that a separate flavonol control mechanism might be at play in pollen.
引用
收藏
页码：985 / 1000
页数：16
相关论文
共 76 条
  • [1] Fruit-Surface Flavonoid Accumulation in Tomato Is Controlled by a SIMYB12-Regulated Transcriptional Network
    Adato, Avital
    Mandel, Tali
    Mintz-Oron, Shira
    Venger, Ilya
    Levy, Dorit
    Yativ, Merav
    Dominguez, Eva
    Wang, Zhonghua
    De Vos, Ric C. H.
    Jetter, Reinhard
    Schreiber, Lukas
    Heredia, Antonio
    Rogachev, Ilana
    Aharoni, Asaph
    [J]. PLOS GENETICS, 2009, 5 (12)
  • [2] Genome-wide Insertional mutagenesis of Arabidopsis thaliana
    Alonso, JM
    Stepanova, AN
    Leisse, TJ
    Kim, CJ
    Chen, HM
    Shinn, P
    Stevenson, DK
    Zimmerman, J
    Barajas, P
    Cheuk, R
    Gadrinab, C
    Heller, C
    Jeske, A
    Koesema, E
    Meyers, CC
    Parker, H
    Prednis, L
    Ansari, Y
    Choy, N
    Deen, H
    Geralt, M
    Hazari, N
    Hom, E
    Karnes, M
    Mulholland, C
    Ndubaku, R
    Schmidt, I
    Guzman, P
    Aguilar-Henonin, L
    Schmid, M
    Weigel, D
    Carter, DE
    Marchand, T
    Risseeuw, E
    Brogden, D
    Zeko, A
    Crosby, WL
    Berry, CC
    Ecker, JR
    [J]. SCIENCE, 2003, 301 (5633) : 653 - 657
  • [3] [Anonymous], 2006, The science of flavonoids
  • [4] TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana
    Baudry, A
    Heim, MA
    Dubreucq, B
    Caboche, M
    Weisshaar, B
    Lepiniec, L
    [J]. PLANT JOURNAL, 2004, 39 (03) : 366 - 380
  • [5] Quercetin: potentials in the prevention and therapy of disease
    Bischoff, Stephan C.
    [J]. CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE, 2008, 11 (06) : 733 - 740
  • [6] Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis
    Borevitz, JO
    Xia, YJ
    Blount, J
    Dixon, RA
    Lamb, C
    [J]. PLANT CELL, 2000, 12 (12) : 2383 - 2393
  • [7] Bowman J., 1994, ARABIDOPSIS ATLAS MO
  • [8] Flavonoids act as negative regulators of auxin transport in vivo in Arabidopsis
    Brown, DE
    Rashotte, AM
    Murphy, AS
    Normanly, J
    Tague, BW
    Peer, WA
    Taiz, L
    Muday, GK
    [J]. PLANT PHYSIOLOGY, 2001, 126 (02) : 524 - 535
  • [9] Flavonoids are differentially taken up and transported long distances in Arabidopsis
    Buer, Charles S.
    Muday, Gloria K.
    Djordjevic, Michael A.
    [J]. PLANT PHYSIOLOGY, 2007, 145 (02) : 478 - 490
  • [10] Ethylene modulates flavonoid accumulation and gravitropic responses in roots of Arabidopsis
    Buer, CS
    Sukumar, P
    Muday, GK
    [J]. PLANT PHYSIOLOGY, 2006, 140 (04) : 1384 - 1396
12345678