Analysis of PRODUCTION OF FLAVONOL GLYCOSIDES-dependent flavonol glycoside accumulation in Arabidopsis thaliana plants reveals MYB11-, MYB12-and MYB111-independent flavonol glycoside accumulation

被引：271

作者：

Stracke, Ralf ^{[1
]}

Jahns, Oliver ^{[1
]}

Keck, Matthias ^{[1
]}

Tohge, Takayuki ^{[2
,3
]}

Niehaus, Karsten ^{[1
]}

Fernie, Alisdair R. ^{[3
]}

Weisshaar, Bernd ^{[1
]}

机构：

[1] Univ Bielefeld, Dept Biol, D-33594 Bielefeld, Germany

[2] RIKEN Plant Sci Ctr, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

[3] Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany

来源：

NEW PHYTOLOGIST | 2010年 / 188卷 / 04期

关键词：

Arabidopsis thaliana; flavonoid biosynthesis; flavonol glycosides; flavonols; gene regulation; R2R3-MYB; UV-B RADIATION; AUXIN TRANSPORT; PHENYLPROPANOID BIOSYNTHESIS; DOMAIN PROTEIN; GENE ENCODES; EXPRESSION; R2R3-MYB; MUTANTS; BIOCHEMISTRY; GROWTH;

D O I：

10.1111/j.1469-8137.2010.03421.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

P>The flavonol branch of flavonoid biosynthesis is under transcriptional control of the R2R3-MYBs PRODUCTION OF FLAVONOL GLYCOSIDE1 (PFG1/MYB12, PFG2/MYB11 and PFG3/MYB111) in Arabidopsis thaliana. Here, we investigated the influence of specific PFG transcription factors on flavonol distribution in various organs. A combination of genetic and metabolite analysis was used to identify transcription factor gene-metabolite correlations of the flavonol metabolic pathway. Flavonol glycoside accumulation patterns have been analysed in wild-type and multiple R2R3-MYB PFG mutants in an organ- and development-dependent manner using high-performance thin-layer chromatography, supplemented with liquid chromatography-mass spectroscopy metabolite profiling. Our results clearly demonstrate a differential influence of MYB11, MYB12 and MYB111 on the spatial accumulation of specific flavonol derivatives in leaves, stems, inflorescences, siliques and roots. In addition, MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation was observed in pollen grains and siliques/seeds. The highly complex tissue- and developmental-specific regulation of flavonol biosynthesis in A. thaliana is orchestrated by at least four PFG transcription factors, differentially influencing the spatial accumulation of specific flavonol derivatives. We present evidence that a separate flavonol control mechanism might be at play in pollen.

引用

页码：985 / 1000

页数：16

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