Hydration dynamics at fluorinated protein surfaces

被引：61
|
作者：
Kwon, Oh-Hoon [1 ,2 ]
Yoo, Tae Hyeon [1 ]
Othona, Christina M. [1 ,2 ]
Van Deventer, James A. [1 ]
Tirrell, David A. [1 ]
Zewail, Ahmed H. [1 ,2 ]
机构：
[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
[2] CALTECH, Phys Biol Ctr Ultrafast Sci & Technol, Arthur Amos Noyes Lab Chem Phys, Pasadena, CA 91125 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2010年 / 107卷 / 40期
基金：
美国国家卫生研究院; 美国国家科学基金会;
关键词：
fluorine; noncanonical amino acids; protein engineering; solvation dynamics; ultrafast hydration; NONCANONICAL AMINO-ACIDS; HUMAN SERUM-ALBUMIN; COILED-COIL; TRYPTOPHAN FLUORESCENCE; CIRCULAR-DICHROISM; SOLVATION DYNAMICS; BUNDLE PROTEIN; WATER DYNAMICS; DRIVING-FORCE; IN-VIVO;
D O I：
10.1073/pnas.1011569107
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Water-protein interactions dictate many processes crucial to protein function including folding, dynamics, interactions with other biomolecules, and enzymatic catalysis. Here we examine the effect of surface fluorination on water-protein interactions. Modification of designed coiled-coil proteins by incorporation of 5,5,5-trifluoroleucine or (4S)-2-amino-4-methylhexanoic acid enables systematic examination of the effects of side-chain volume and fluorination on solvation dynamics. Using ultrafast fluorescence spectroscopy, we find that fluorinated side chains exert electrostatic drag on neighboring water molecules, slowing water motion at the protein surface.
引用
收藏
页码：17101 / 17106
页数：6
相关论文
共 50 条
  • [1] How protein surfaces induce anomalous dynamics of hydration water
    Pizzitutti, Francesco
    Marchi, Massimo
    Sterpone, Fabio
    Rossky, Peter J.
    JOURNAL OF PHYSICAL CHEMISTRY B, 2007, 111 (26): : 7584 - 7590
  • [2] PROTEIN DYNAMICS AND HYDRATION
    FRAUENFELDER, H
    GRATTON, E
    METHODS IN ENZYMOLOGY, 1986, 127 : 207 - 216
  • [3] Aqueous peptides as experimental models for hydration water dynamics near protein surfaces
    Malardier-Jugroot, Cecile
    Johnson, Margaret E.
    Murarka, Rajesh K.
    Head-Gordon, Teresa
    PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2008, 10 (32) : 4903 - 4908
  • [4] Water hydration in protein dynamics
    Bellissent-Funel, Marie-Claire
    BIOFUTUR, 2012, (331) : 31 - 33
  • [5] DYNAMICS OF HYDRATION WATER IN PROTEIN
    BELLISSENTFUNEL, MC
    TEIXEIRA, J
    BRADLEY, KF
    CHEN, SH
    JOURNAL DE PHYSIQUE I, 1992, 2 (06): : 995 - 1001
  • [6] Dynamics of protein hydration water
    Wolf, M.
    Emmert, S.
    Gulich, R.
    Lunkenheimer, P.
    Loidl, A.
    PHYSICAL REVIEW E, 2015, 92 (03):
  • [7] Protein dynamics: hydration and cavities
    Heremans, K
    BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, 2005, 38 (08) : 1157 - 1165
  • [8] Dynamics of protein and peptide hydration
    Modig, K
    Liepinsh, E
    Otting, G
    Halle, B
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (01) : 102 - 114
  • [9] Hydration in protein dynamics and function
    Bellissent-Funel, MC
    JOURNAL OF MOLECULAR LIQUIDS, 2000, 84 (01) : 39 - 52
  • [10] PHYS 327-Hydration dynamics at protein and DNA surfaces: Mechanism of orientational and hydrogen bond breaking dynamics
    Jana, B.
    Pal, S.
    Bagchi, B.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2008, 235
12345