Detecting the expression of human endogenous retrovirus E envelope transcripts in human prostate adenocarcinoma

被引:76
|
作者
Wang-Johanning, F
Frost, AR
Jian, B
Azuero, R
Lu, DW
Chen, DT
Johanning, GL
机构
[1] Univ Alabama Birmingham, Dept Nutr Sci, Birmingham, AL 35294 USA
[2] Univ Alabama Birmingham, Dept Med, Birmingham, AL 35294 USA
[3] Univ Alabama Birmingham, Dept Pathol, Birmingham, AL 35294 USA
[4] Washington Univ, Med Ctr, Lauren V Ackerman Lab Surg Pathol, St Louis, MO 63130 USA
[5] Univ Alabama Birmingham, Biostat & Bioinformat Unit, Birmingham, AL 35294 USA
关键词
prostate carcinoma; human endogenous retrovirus; envelope proteins; novel tumor markers;
D O I
10.1002/cncr.11451
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
BACKGROUND. The expression of human endogenous retrovirus (HERV) mRNA and proteins was associated recently with diseases that include human malignancies. The authors report that, in the current study, transcripts encoding the envelope region of an HERV family, HERV-E, were expressed in human prostate carcinoma. METHODS. RNA was isolated from various prostate tissues and was tested for the expression of various HERV envelope (env) genes by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, RNA in situ hybridization (ISH), and Northern blot analysis. Variants of HERV that appeared in prostate carcinoma tissues were sequenced, and HERV-E was expressed in prokaryotic and eukaryotic systems. RESULTS. In the current study, the authors found that the mRNA of the env gene of one particular family of FIERVs, HERV-E, was expressed in some prostate carcinoma tissues (38.8% positive; n = 49 specimens) but not in normal prostate tissues using RT-PCR, RNA ISH, and Northern blot assays. The expression of FIERV-E transcripts in prostate tumor epithelial cells was confirmed further by ISH using an HERV-E specific antisense probe. Approximately 50% of the cDNA of HERV-E obtained from prostate carcinoma specimens contained no stop codon and expressed proteins in prokaryotic or eukaryotic expression systems. Furthermore, the expression of both FIERV-E and ERV3 (another class of HERV) was detected in the same prostate carcinoma tissues. CONCLUSIONS. The expression and distribution of multiple HERV-E endogenous retroviral elements in prostate carcinoma, but not in normal control specimens, suggests that they may serve as novel tumor markers for the early diagnosis and immunotherapy of patients with prostate carcinoma. Cancer 2003;98:187-97. (C) 2003 American Cancer Society.
引用
收藏
页码:187 / 197
页数:11
相关论文
共 50 条
  • [21] The envelope of human endogenous retrovirus mediates neuro-inflammation in mice
    Reynaud-Dougier, H-L
    Lomparski, C.
    Villiers, C. L.
    Jouvin-Marche, E.
    Perron, H.
    Marche, P. N.
    MULTIPLE SCLEROSIS JOURNAL, 2012, 18 : 351 - 352
  • [22] Differential expression of human endogenous retrovirus K transcripts in primary human melanocytes and melanoma cell lines after UV irradiation
    Reiche, Janine
    Pauli, Georg
    Ellerbrok, Heinz
    MELANOMA RESEARCH, 2010, 20 (05) : 435 - 440
  • [23] Expression of envelope proteins of endogenous C-type retrovirus on the surface of mouse and human oocytes at fertilization
    Nilsson, O
    Jin, M
    Andersson, AC
    Sundström, P
    Larsson, E
    HUMAN REPRODUCTION, 1998, 13 : 209 - 210
  • [24] ABSENCE OF EXPRESSION OF A HUMAN ENDOGENOUS RETROVIRUS IS CORRELATED WITH CHORIOCARCINOMA
    KATO, N
    LARSSON, E
    COHEN, M
    INTERNATIONAL JOURNAL OF CANCER, 1988, 41 (03) : 380 - 385
  • [25] Expression of human endogenous retrovirus K and W in babies
    Nali, L. H. S.
    Oliveira, A. C. S.
    Alves, D. O.
    Caleiro, G. S.
    Nunes, C. F.
    Gerhardt, D.
    Succi, R. C. M.
    Romano, Camila M.
    Machado, D. M.
    ARCHIVES OF VIROLOGY, 2017, 162 (03) : 857 - 861
  • [26] Expression and regulation of human endogenous retrovirus W elements
    Li, Fang
    Karlsson, Hakan
    APMIS, 2016, 124 (1-2) : 52 - 66
  • [27] Human endogenous retrovirus (HERV)-K transcripts in germ cell and trophoblastic tumours
    Herbst, H
    Sauter, M
    Kuhler-Obbarius, C
    Loning, T
    Mueller-Lantzsch, N
    APMIS, 1998, 106 (01) : 216 - 220
  • [28] Expression of human endogenous retrovirus K and W in babies
    L. H. S. Nali
    A. C. S. Oliveira
    D. O. Alves
    G. S. Caleiro
    C. F. Nunes
    D. Gerhardt
    R. C. M. Succi
    Camila M. Romano
    D. M. Machado
    Archives of Virology, 2017, 162 : 857 - 861
  • [29] Locus-Specific Characterization of Human Endogenous Retrovirus Expression in Prostate, Breast, and Colon Cancers
    Steiner, Margaret C.
    Marston, Jez L.
    Iniguez, Luis P.
    Bendall, Matthew L.
    Chiappinelli, Katherine B.
    Nixon, Douglas F.
    Crandall, Keith A.
    CANCER RESEARCH, 2021, 81 (13) : 3449 - 3460
  • [30] Human endogenous retrovirus-W envelope abundance and infectivity in multiple sclerosis
    Antony, J
    Izad, M
    Zhang, K
    Warren, K
    Vodjgani, M
    Power, C
    JOURNAL OF NEUROIMMUNOLOGY, 2004, 154 (1-2) : 185 - 185