Octanoyl-homoserine lactone is the cognate signal for Burkholderia mallei BmaR1-BmaI1 quorum sensing

被引:39
作者
Duerkop, Breck A.
Ulrich, Ricky L.
Greenberg, E. Peter
机构
[1] Univ Washington, Sch Med, Dept Microbiol, Seattle, WA 98195 USA
[2] USA, Med Res Inst Infect Dis, Bacteriol Div, Ft Detrick, MD 21704 USA
关键词
D O I
10.1128/JB.00317-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Acyl-homoserine lactones (HSLs) serve as quorum-sensing signals for many Proteobacteria. Members of the LuxI family of signal generators catalyze the production of acyl-HSLs, which bind to a cognate receptor in the LuxR family of transcription factors. The obligate animal pathogen Burkholderia mallei produces several acyl-HSLs, and the B. mallei genome has four lurR and two luxI homologs, each of which has been established as a virulence factor. To begin to delineate the relevant acyl-HSL signals for B. mallei LuxR homologs, we analyzed the BmaR1-BmaI1 system. A comparison of acyl-HSL profiles from B. mallei ATCC 23344 and a B. mallei bmaI1 mutant indicates that octanoyl-HSL synthesis is BmaI1 dependent. Furthermore, octanoyl-HSL is the predominant acyl-HSL produced by BmaI1 in recombinant Escherichia coli. The synthesis of soluble BmaR1 in recombinant E. coli requires octanoyl-HSL or decanoyl-HSL. Insoluble aggregates of BmaR1 are produced in the presence of other acyl-HSLs and in the absence of acyl-HSLs. The bmaI1 promoter is activated by BmaR1 and octanoyl-HSL, and a 20-bp inverted repeat in the bmaI1 promoter is required for bmaI1 activation. Purified BmaR1 binds to this promoter region. These findings implicate octanoyl-HSL as the signal for Bmalk1-BmaI1 quorum sensing and show that octanoyl-HSL and BmaR1 activate bmaT1 transcription.
引用
收藏
页码:5034 / 5040
页数:7
相关论文
共 45 条
[1]  
Aiyar A, 1996, Methods Mol Biol, V57, P177
[2]  
BERINGER JE, 1974, J GEN MICROBIOL, V84, P188
[3]   Characterization of Burkholderia pseudomallei and Burkholderia pseudomallei-like strains [J].
Brett, PJ ;
Deshazer, D ;
Woods, DE .
EPIDEMIOLOGY AND INFECTION, 1997, 118 (02) :137-148
[4]   Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species [J].
Brett, PJ ;
DeShazer, D ;
Woods, DE .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 :317-320
[5]   SYNTHESIS OF MULTIPLE EXOPRODUCTS IN PSEUDOMONAS-AERUGINOSA IS UNDER THE CONTROL OF RHLR-RHLI, ANOTHER SET OF REGULATORS IN STRAIN PAO1 WITH HOMOLOGY TO THE AUTOINDUCER-RESPONSIVE LUXR-LUXI FAMILY [J].
BRINT, JM ;
OHMAN, DE .
JOURNAL OF BACTERIOLOGY, 1995, 177 (24) :7155-7163
[6]   QscR, a modulator of quorum-sensing signal synthesis and virulence in Pseudomonas aeruginosa [J].
Chugani, SA ;
Whiteley, M ;
Lee, KM ;
D'Argenio, D ;
Manoil, C ;
Greenberg, EP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (05) :2752-2757
[7]   Diversity and significance of Burkholderia species occupying diverse ecological niches [J].
Coenye, T ;
Vandamme, P .
ENVIRONMENTAL MICROBIOLOGY, 2003, 5 (09) :719-729
[8]   Quorum-sensing signals and quorum-sensing genes in Burkholderia vietnamiensis [J].
Conway, BA ;
Greenberg, EP .
JOURNAL OF BACTERIOLOGY, 2002, 184 (04) :1187-1191
[9]  
DESHAZER D, 2005, BIOL WEAPONS DEFENSE, P209
[10]   IDENTIFICATION OF THE OPERATOR OF THE LUX REGULON FROM THE VIBRIO-FISCHERI STRAIN ATCC7744 [J].
DEVINE, JH ;
SHADEL, GS ;
BALDWIN, TO .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (15) :5688-5692