Effect of Semax peptide on survival of cultured rat pheochromocytoma cells during oxidative stress

被引：5

作者：

Safarova, ER ^{[1
]}

Shram, SI ^{[1
]}

Zolotarev, YA ^{[1
]}

Myasoedov, NF ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Mol Genet, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2003年 / 135卷 / 03期

关键词：

brain ischemia; oxidative stress; necrosis; Semax; cell culture; rat pheochromocytoma PC12;

D O I：

10.1023/A:1024141232307

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We studied the effects of Semax (antiinsulin peptide with neuroprotective effect) on the survival of cultured rat pheochromocytoma cell after oxidative stress induced by short-term incubation with hydrogen peroxide. Studies with fluorescent dyes propidium iodide and Hoechst 33258 showed that cell incubation with hydrogen peroxide led to the formation of damaged cells with characteristic signs of necrosis. Semax dose-dependently reduced the number of cells damaged by oxidative stress. The efficiency of Semax depended on the time of its addition to the culture medium. The results suggest that the neuroprotective effect of Semax in ischemic stroke can be due to its capacity to protect neurons from damage caused by oxidative stress.

引用

页码：268 / 271

页数：4

共 14 条

[1]

Ashmarin IP, 1997, ZH VYSSH NERV DEYAT+, V47, P420

[2] Novel synthetic analogue of ACTH 4-10 (Semax) but not glycine prevents the enhanced nitric oxide generation in cerebral cortex of rats with incomplete global ischemia [J].

Bashkatova, VG ;

Koshelev, VB ;

Fadyukova, OE ;

Alexeev, AA ;

Vanin, AF ;

Rayevsky, KS ;

Ashmarin, IP ;

Armstrong, DM .

BRAIN RESEARCH, 2001, 894 (01) :145-149

[3]

DOLOTOV OV, 2000, PROSP PHYS BIOL P WO, V1, P236

[4]

Grivennikov IA, 1999, MOL BIOL+, V33, P103

[5]

Gusev EI, 1997, ZH NEVROPATOL PSIKH, V97, P26

[6]

Gusev EI, 2001, BRAIN ISCHEMIA

[7] A CELLULAR-MODEL OF OXIDANT-MEDIATED NEURONAL INJURY [J].

HINSHAW, DB ;

MILLER, MT ;

OMANN, GM ;

BEALS, TF ;

HYSLOP, PA .

BRAIN RESEARCH, 1993, 615 (01) :13-26

[8]

Kaplan A. Ya., 1992, Fiziologiya Cheloveka, V18, P104

[9] Ischemic cell death in brain neurons [J].

Lipton, P .

PHYSIOLOGICAL REVIEWS, 1999, 79 (04) :1431-1568

[10]

Love S, 1999, BRAIN PATHOL, V9, P119

← 1 2 →