Synthesize of Bi2O3/Gln-TSC nanoparticles and evaluation of their toxicity on prostate cancer cells and expression of CASP8, BAX, and Bcl-2 genes

被引：8

作者：

Moradi, Asal ^{[1
]}

Abdihaji, Mohammadreza ^{[2
]}

Kouchaksaraie, Sara Barari ^{[1
]}

Alkinani, Tabarek Abdulrazaq ^{[3
]}

Mahmoudi, Aida ^{[4
]}

Davoudi, Arash ^{[1
,5
]}

Dashtmiani, William ^{[6
]}

Ghezeljeh, Somayeh Mikaeili ^{[1
]}

Aghajani, Shahrzad ^{[1
]}

Ghasemian, Reza ^{[7
]}

Taramsari, Somayeh Maghsoomi ^{[8
]}

Majlesi, Amitis ^{[1
]}

Niyaki, Zahra Mahdavi ^{[1
]}

Salehzadeh, Ali ^{[1
]}

机构：

[1] Islamic Azad Univ, Dept Biol, Rasht Branch, Rasht, Iran

[2] Indiana Univ, Ctr Genom & Bioinformat, Dept Biol, Bloomington, IN USA

[3] Islamic Azad Univ, Dept Biol, Sci & Res Branch, Tehran, Iran

[4] Islamic Azad Univ, Dept Biol, Damghan Branch, Damghan, Iran

[5] Dr Keshavarz Med Genet Lab, Div Cytogenet, Rasht, Iran

[6] Univ New South Wales, St George Hosp, Dept Surg, Canc Res Labs, Sydney, NSW 2217, Australia

[7] Islamic Azad Univ, Fac Med, Dept Med Sci, Tehran Med Sci, Tehran, Iran

[8] Univ Vienna, Max Perutz Labs, Vienna Bioctr VBC, Dr Bohr Gasse 9, Vienna, Austria

来源：

SCIENTIFIC REPORTS | 2022年 / 12卷 / 01期

基金：

英国科研创新办公室;

关键词：

APOPTOSIS; CASPASE-8; MECHANISM;

D O I：

10.1038/s41598-022-25360-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Due to the high prevalence and considerable increase of prostate cancer, finding novel therapeutic compounds for the treatment of prostatic cancer has been the goal of many researches. In this study, we aimed to fabricate the Bismuth oxide (Bi2O3) NPs, functionalized with glutamine (Gln) and conjugated with Thiosemicarbazide (TSC). Then, the anticancer mechanism of the synthesized NPs was investigated using the cellular and molecular tests including MTT assay, Flow cytometry, Caspase-3 activity assay, Hoechst staining and Real Time PCR. The FT-IR and XRD assays confirmed the identity of the synthesized Bi2O3/Gln-TSC NPs. The size range of the synthesized spherical particles was 10-60 nm and the zeta potential was - 23.8 mV. The purity of the NPs was confirmed by EDX-mapping analysis. The Bi2O3/Gln-TSC was considerably more toxic for prostate cancer cells than normal human cells and the IC50 was calculated 35.4 and 305 mu g/mL, respectively. The exposure to the NPs significantly increased the frequency of apoptotic cells from 4.7 to 75.3%. Moreover, the expression of the CASP8, BAX, and Bcl-2 genes after exposure to the NPs increased by 2.8, 2.3, and 1.39 folds. Treating the cancer cells with Bi2O3/Gln-TSC increased the activity of the Caspase-3 protein and apoptotic morphological features were observed by Hoechst staining in the treated cells. This work showed that Bi2O3/Gln-TSC has considerable cytotoxicity for prostate cancer cells and could inducing both intrinsic and extrinsic pathways of apoptosis.

引用

页数：14

共 37 条

[1] Preparation of Novel Nickel Oxide@ Glutamic/Thiosemicarbazide Nanoparticles: Implications for Cytotoxic and Anti-cancer Studies in MCF-7 Breast Cancer Cells [J].

Abbaszadeh, Niloufar ;

Jaahbin, Negar ;

Pouraei, Ayda ;

Mehraban, Fatemeh ;

Hedayati, Mohammad ;

Majlesi, Amitis ;

Akbari, Fatemeh ;

Sadat Shandiz, Seyed Ataollah ;

Salehzadeh, Ali .

JOURNAL OF CLUSTER SCIENCE, 2022, 33 (02) :457-465

[2] Facile electrosynthesis and characterization of superparamagnetic nanoparticles coated with cysteine, glycine and glutamine [J].

Aghazadeh, Mustafa ;

Karimzadeh, Isa ;

Doroudi, Taher ;

Ganjali, Mohammad Reza ;

Kolivand, Peir Hossein ;

Gharailou, Davoud .

APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, 2017, 123 (08)

[3] Oxidative stress mediated cytotoxicity and apoptosis response of bismuth oxide (Bi2O3) nanoparticles in human breast cancer (MCF-7) cells [J].

Ahamed, Maqusood ;

Akhtar, Mohd Javed ;

Khan, M. A. Majeed ;

Alrokayan, Salman A. ;

Alhadlaq, Hisham A. .

CHEMOSPHERE, 2019, 216 :823-831

[4] Bismuth oxide nanoparticles induce oxidative stress and apoptosis in human breast cancer cells [J].

Alamer, Ali ;

Ali, Daoud ;

Alarifi, Saud ;

Alkahtane, Abdullah ;

AL-Zharani, Mohammed ;

Abdel-Daim, Mohamed M. ;

Albasher, Gadah ;

Almeer, Rafa ;

Al-Sultan, Nouf K. ;

Almalik, Abdulaziz ;

Alhasan, Ali H. ;

Stournaras, Christos ;

Hasnain, Saquib ;

Alkahtani, Saad .

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2021, 28 (06) :7379-7389

[5] Synthesis, Characterization, and in Vitro Anticancer Activity of Copper and Zinc Bis(Thiosemicarbazone) Complexes [J].

Anjum, Rukhsana ;

Palanimuthu, Duraippandi ;

Kalinowski, Danuta S. ;

Lewis, William ;

Park, Kyung Chan ;

Kovacevic, Zaklina ;

Khan, Irfan Ullah ;

Richardson, Des R. .

INORGANIC CHEMISTRY, 2019, 58 (20) :13709-13723

[6] Preferential glutamine uptake in cancer cells [J].

不详 .

NATURE REVIEWS NEPHROLOGY, 2021, 17 (6) :368-368

[7] Nanoparticles in Cancer Treatment: Opportunities and Obstacles [J].

Awasthi, Rajendra ;

Roseblade, Ariane ;

Hansbro, Philip Michael ;

Rathbone, Michael John ;

Dua, Kamal ;

Bebawy, Mary .

CURRENT DRUG TARGETS, 2018, 19 (14) :1696-1709

[8] Assessment of the Antibacterial Effects of Bismuth Nanoparticles against Enterococcus faecalis [J].

Azad, Azita ;

Rostamifar, Sahar ;

Modaresi, Farzan ;

Bazrafkan, Ali ;

Rezaie, Zahra .

BIOMED RESEARCH INTERNATIONAL, 2020, 2020

[9] Cytotoxic and apoptotic properties of silver chloride nanoparticles synthesized using Escherichia coli cell-free supernatant on human breast cancer MCF 7 cell line [J].

Bigdeli, Razieh ;

Shahnazari, Masoumeh ;

Panahnejad, Erfan ;

Cohan, Reza Ahangari ;

Dashbolaghi, Aziz ;

Asgary, Vahid .

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, 2019, 47 (01) :1603-1609

[10] Functionalization of silver nanoparticles with glutamine and histidine for simple and selective detection of Hg2+ ion in water samples [J].

Buduru, Prasad ;

Reddy, B. C. Sundher Raja ;

Naidu, N. V. S. .

SENSORS AND ACTUATORS B-CHEMICAL, 2017, 244 :972-982

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