Anti-angiogenic effects of ethanolic extract of Artemisia sieberi compared to its active substance, artemisinin

被引:21
作者
Abdolmaleki, Zohreh [1 ]
Arab, Hossein-Ali [1 ]
Amanpour, Saeid [2 ]
Muhammadnejad, Samad [2 ]
机构
[1] Univ Tehran, Fac Vet Med, Dept Pharmacol, Tehran, Iran
[2] Univ Tehran Med Sci, Tumor Model Res Ctr, Canc Inst Iran, Tehran, Iran
来源
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY | 2016年 / 26卷 / 03期
关键词
Angiogenesis; Human umbilical endothelial cells; Chick chorioallantoic membrane; Artemisia sieberi; Artemisinin; ENDOTHELIAL GROWTH-FACTOR; TUMOR ANGIOGENESIS; ANTIANGIOGENIC ACTIVITY; INHIBITS ANGIOGENESIS; SESQUITERPENE LACTONE; CLINICAL-APPLICATIONS; ANTIMALARIAL-DRUG; FACTOR EXPRESSION; ARTESUNATE; CANCER;
D O I
10.1016/j.bjp.2015.11.008
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Angiogenesis plays a key role in tumor growth, invasion and metastasis of cancer diseases and therefore, the inhibition of angiogenesis can provide an important therapeutic approach in cancer diseases. This study was designed to compare the anti-angiogenic activities of the ethanolic extract of Artemisia sieberi Besser, Asteraceae, and its active substance, artemisinin in both in vitro and in vivo models. To compare cytotoxicity level of ethanolic extract of A. sieberi with artemisinin, different concentrations (1100 mu g/ml) were tested using MTT assay on human umbilical vein endothelial cells. The anti-angiogenic properties of serial concentrations of ethanolic extract of A. sieberi and artemisinin were examined on human umbilical vein endothelial cells using a three-dimensional angiogenesis assay (in vitro model) and in the chick chorioallantoic membrane assay as in vivo model. The effects of ethanolic extract of A. sieberi and artemisinin were also tested on the expression of VEGFR-1, VEGFR-2 and CD34 genes using real-time PCR. Ethanolic extract of A. sieberi and artemisinin significantly (p < 0.001) inhibited the angiogenesis in the human umbilical vein endothelial cells culture whilst the ethanolic extract of A. sieberi showed higher effect in a concentration-dependent fashion (p < 0.001). The chick chorioallantoic membrane angiogenesis was also completely inhibited by ethanolic extract of A. sieberi at concentration of 33 ng/100 mu l/egg. The gene expression analysis showed that the ethanolic extract of A. sieberi and artemisinin reduced the transcription of VEGFR-1, VEGFR-2 and CD34 genes in a concentration-dependent manner. This study demonstrated that the ethanolic extract of A. sieberi is strongly able to inhibit the angiogenesis in human umbilical vein endothelial cells and chick chorioallantoic membrane models compared to the artemisinin. (C) 2016 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. All rights reserved.
引用
收藏
页码:326 / 333
页数:8
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