Overview of DNA chip technology

被引：133

作者：

Lemieux, B

Aharoni, A

Schena, M ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Beckman Ctr, Dept Biochem, Stanford, CA 94305 USA

[2] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19717 USA

[3] DLO, Ctr Plant Breeding & Reprod Res, Dept Cell Biol, NL-6700 AA Wageningen, Netherlands

来源：

MOLECULAR BREEDING | 1998年 / 4卷 / 04期

关键词：

genomics; genotyping; microarrays; mutation detection; transcript monitoring;

D O I：

10.1023/A:1009654300686

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

DNA chip technology utilizes microscopic arrays (microarrays) of molecules immobilized on solid surfaces for biochemical analysis. Microarrays can be used for expression analysis, polymorphism detection, DNA resequencing, and genotyping on a genomic scale. Advanced arraying technologies such as photolithograpy, micro-spotting and ink jetting, coupled with sophisticated fluorescence detection systems and bioinformatics, permit molecular data gathering at an unprecedented rate. Microarray-based characterization of plant genomes has the potential to revolutionize plant breeding and agricultural biotechnology. This review provides an overview of DNA chip technology, focusing on manufacturing approaches and biological applications.

引用

页码：277 / 289

页数：13

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