A preliminary study of the use of larch arabinogalactan in aqueous two-phase systems

被引:12
作者
Christian, TJ
Manley-Harris, M
Richards, GN
机构
[1] Univ Waikato, Dept Chem, Hamilton, New Zealand
[2] Univ Montana, Shafizadeh Ctr Wood & Carbohydrate Chem, Missoula, MT 59812 USA
关键词
D O I
10.1016/S0144-8617(97)00243-9
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The polysaccharide arabinogalactan (AG) can provide a low cost alternative to the fractionated dextrans for use with polyethylene glycol (PEG) in aqueous two-phase, two-polymer systems. The narrow molecular weight range and low viscosity at high concentration of AG provide for predictable and reproducible separations under a variety of conditions. The partition behavior of bovine serum albumin (BSA) was studied under varying conditions of pH, AG:PEG concentration ratios, and PEG molecular weights. Batch extractive bioconversions of corn starch to cyclodextrin and glucose were demonstrated using AG-PEG systems. (C) 1998 Elsevier Science Limited. All rights reserved.
引用
收藏
页码:7 / 12
页数:6
相关论文
共 27 条
[1]  
Albertsson P.A., 1986, PARTITION CELL PARTI
[2]  
Antonov Yu. A., 1982, Carbohydrate Polymers, V2, P81, DOI 10.1016/0144-8617(82)90054-6
[3]  
Armbruster FC, 1988, P 4 INT S CYCL, P33
[4]  
Birkenmeier G, 1986, Biomed Chromatogr, V1, P64, DOI 10.1002/bmc.1130010205
[5]  
CLEWLOW AC, 1995, BIOPOLYMER MIXTURES, P1873
[6]  
DEA I.C.M, 1993, Industrial Gums, P21, DOI 10.1016/B978-0-08-092654-4.50006-1
[7]  
DOUMAS BT, 1981, CLIN CHEM, V27, P1642
[8]   ENZYMATIC-HYDROLYSIS OF SOLUBLE STARCH IN A POLYETHYLENE GLYCOL-DEXTRAN AQUEOUS 2-PHASE SYSTEM [J].
HAYASHIDA, K ;
KUNIMOTO, K ;
SHIRAISHI, F ;
KAWAKAMI, K ;
ARAI, Y .
JOURNAL OF FERMENTATION AND BIOENGINEERING, 1990, 69 (04) :240-243
[9]   PARTITION OF PROTEINS AND MICROORGANISMS IN AQUEOUS BIPHASIC SYSTEMS [J].
JOHANSSON, G .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 1974, 4 (03) :169-180
[10]   AFFINITY PARTITIONING OF PHOSPHOFRUCTOKINASE FROM BAKERS-YEAST USING POLYMER-BOUND CIBACRON BLUE F3G-A [J].
JOHANSSON, G ;
KOPPERSCHLAGER, G ;
ALBERTSSON, PA .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 131 (03) :589-594