Less is more - length reduction of STR amplicons using redesigned primers

被引：96

作者：

Wiegand, P

Kleiber, M

机构：

[1] Univ Ulm, Dept Legal Med, Inst Pathol & Legal Med, D-89075 Ulm, Germany

[2] Univ Halle Wittenberg, Inst Legal Med, D-06112 Halle, Germany

来源：

INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 2001年 / 114卷 / 4-5期

关键词：

STRs; primers; degradation; DNA;

D O I：

10.1007/s004140000162

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

PCR primers closely flanking the repeat region were redesigned to reduce the amplicon length of the selected STRs down to approximately 100 bp for the shorter alleles (loci HumTH01, D10S2325, DYS19 and DYS391). Highly degraded DNA (e.g. formalin-fixed tissue) and very low amounts of DNA could be more successfully typed using the new redesigned primers compared to the established sequences generating longer amplicons.

引用

页码：285 / 287

页数：3

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