Sandwich ELISA and immunochromatographic strip of Kunitz trypsin inhibitor using sensitive monoclonal antibodies

被引：14

作者：

Xu, Liguang ^{[1
,2
]}

Song, Yuehong ^{[1
,2
]}

Liu, Liqiang ^{[1
,2
]}

Song, Shanshan ^{[1
,2
]}

Zhu, Jianping ^{[1
,2
]}

Kuang, Hua ^{[1
,2
]}

Xu, Chuanlai ^{[1
,2
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2016年 / 27卷 / 06期

关键词：

Kunitz trypsin inhibitor; sandwich ELISA; immunochromatographic strip; soybean; monoclonal antibody; BOWMAN-BIRK INHIBITOR; CHROMATOGRAPHY; PURIFICATION; IMMUNOASSAY; RESIDUES; MILK; FOOD;

D O I：

10.1080/09540105.2016.1160367

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Kunitz trypsin inhibitor (KTI) is a predominant anti-nutritional factor in soybean. The objective of this study was to develop a sandwich enzyme-linked immunosorbent assay and immunochromato graphic strip (ICS) method for the semi-quantification of KTI in soybean. The limit of detection was 0.1481ng/ml, and the linear dynamic range was 0.2-20ng/ml. KTI recovery was 98.40-110.66% from KTI-spiked milk samples, with coefficients of variation of 4.41-10.62%. Based on ICS results, KTI had cutoff values of 5ng/ml and 2.5ng/ml in 0.05% Tween-added phosphate-buffered saline and milk, respectively. ICS has potential applications for the fast detection of KTI.

引用

页码：772 / 782

页数：11

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