Hsa-miRNA-143-3p Reverses Multidrug Resistance of Triple-Negative Breast Cancer by Inhibiting the Expression of Its Target Protein Cytokine-Induced Apoptosis Inhibitor 1 In Vivo

被引:23
作者
Deng, Yu Wei [1 ]
Hao, Wen Jing [1 ]
Li, Yi Wen [1 ]
Li, Yi Xin [1 ]
Zhao, Bo Chen [1 ]
Lu, Dan [1 ]
机构
[1] Harbin Med Univ, Affiliated Hosp 2, Dept Med Oncol, 246 Xuefu Rd, Harbin 150086, Heilongjiang, Peoples R China
关键词
Cytokine-induced apoptosis inhibitor 1; Human mammaglobin; Micro-ribonucleic acid-143-3p; Multiple chug resistance; Triple negative beast neoplasms; DRUG-RESISTANCE; CELL-PROLIFERATION; UP-REGULATION; MICRORNA-143; CHEMORESISTANCE; MECHANISMS; PACLITAXEL; VECTORS; MIR-143; P53;
D O I
10.4048/jbc.2018.21.e40
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Purpose: Multidrug resistance (MDR) remains a major obstacle in the treatment of triple-negative breast cancer (TNBC) with conventional chemotherapeutic agents. A previous study demonstrated that hsa-miRNA-143-3p plays a vital role in drug resistance of TNBC. Downregulation of hsa-miRNA-143-3p upregulated the expression of its target protein cytokine-induced apoptosis inhibitor 1 (CIAPIN1) in order to activate MDR, while upregulation of hsa-miRNA-143-3p effectively enhances the sensitivity of drug-resistant TNBC cells to chemotherapeutics. The present study aimed to further verify these findings in vivo. Methods: We established a hypodermic tumor nude mice model using paclitaxel-resistant TNBC cells. We expressed ectopic hsa-miRNA-143-3p under the control of a breast cancer-specific human mammaglobin promoter that guided the efficient expression of exogenous hsa-miRNA-143-3p only in breast cancer cells. Thereafter, we overexpressed hsa-miRNA-143-3p in xenografts using a recombinant virus system and quantified the expression of hsa-miRNA-143-3p, CIAPIN1 protein, and proteins encoded by related functional genes by western blot. Results: We successfully completed the prospective exploration of the intravenous virus injection pattern from extensive expression to targeted expression. The overexpression of hsa-miRNA-143-3p significantly alleviated chemoresistance of TNBC by inhibiting viability. In addition, we observed that the expression of CIAPIN1 as a hsa-miRNA-143-3p target protein was remarkably decreased. Conclusion: We partly illustrated the mechanism underlying the hsa-miRNA-143-3p/CIAPIN1 drug resistance pathway. HsamiRNA-143-3p as a tumor suppressive microRNA may be a novel target to effectively reverse MDR of TNBC in vivo.
引用
收藏
页码:251 / 258
页数:8
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