Nisin and lysostaphin activity against preformed biofilm of Staphylococcus aureus involved in bovine mastitis

被引:64
作者
Ceotto-Vigoder, H. [1 ,3 ]
Marques, S. L. S. [1 ]
Santos, I. N. S. [1 ]
Alves, M. D. B. [1 ]
Barrias, E. S. [2 ]
Potter, A. [1 ]
Alviano, D. S. [1 ]
Bastos, M. C. F. [1 ]
机构
[1] Univ Fed Rio de Janeiro, Dept Microbiol Geral, Inst Microbiol Paulo de Goes, Ave Carlos Chagas Filho,373 CCS,Bloco 1, BR-21941902 Rio De Janeiro, RJ, Brazil
[2] Inst Nacl Metrol Qualidade & Tecnol, Duque De Caxias, Brazil
[3] Inst Fed Educ Ciencia & Tecnol Rio de Janeiro, Rio De Janeiro, Brazil
关键词
antimicrobials; biofilms; bovine mastitis; lysostaphin; nisin; Staphylococcus aureus; BACTERIOCINS; STRAINS; BAP; POLYSACCHARIDE; PEPTIDES; COW;
D O I
10.1111/jam.13136
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
AimsThe biofilm produced by Staphylococcus aureus isolates involved in clinical or subclinical bovine mastitis and the activity of nisin and lysostaphin against the preformed biofilm produced by these strains were investigated. Methods and ResultsEighteen strains were tested and all produced biofilm. Eight strains with distinct biofilm composition were selected for the antimicrobial activity assays. The minimal inhibitory concentration of each bacteriocin was determined against the planktonic cells and ranged from 156 to 500gml(-1) for nisin, and from 39 to 50gml(-1), for lysostaphin. Lysostaphin treatment (04gml(-1)) for 4h caused a strong Staph.aureus 4181 biofilm detachment and death of the majority of the sessile cells, while nisin treatment (100gml(-1)) for the same time caused only a great reduction in cell viability. Additionally, combination of both bacteriocins for 4h resulted in significant death of the sessile cells but no biofilm detachment. ConclusionsThe treatment with lysostaphin alone or in combination with nisin was effective in killing most biofilm sessile cells. Significance and Impact of the StudyThe action of lysostaphin, either alone or in combination with nisin, against established staphylococcal biofilm may represent an alternative to bovine mastitis control. However, the duration of the treatment should be considered for its application so that the best effectiveness can be achieved.
引用
收藏
页码:101 / 114
页数:14
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