Homogenization with heat treatment: A cost effective alternative to nucleic acid extraction for herpes simplex virus real-time PCR from viral swabs

被引：4
作者：
LeBlanc, Jason J. [1 ,2 ]
Heinstein, Charles R. [1 ]
Hatchette, Todd F. [1 ,2 ]
机构：
[1] Queen Elizabeth 2 Hlth Sci Ctr, Dept Pathol & Lab Med, Halifax, NS, Canada
[2] Dalhousie Univ, Dept Pathol, Halifax, NS, Canada
来源：
JOURNAL OF VIROLOGICAL METHODS | 2012年 / 179卷 / 01期
关键词：
Homogenization; Extraction; Silica beads; PCR inhibitors; Real-time PCR; Herpes simplex virus (HSV); RAPID EXTRACTION; AMPLIFICATION; QUANTITATION; INHIBITION; SAMPLES;
D O I：
10.1016/j.jviromet.2011.09.018
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Most laboratories use expensive commercial kits to purify nucleic acids and remove PCR inhibitors that maybe present in clinical specimens. In this study a simple homogenization with heat treatment of herpes simplex virus types 1 and 2 (HSV-1/2) was shown to be equivalent to commercial kit-based nucleic acid extraction methods. With a cost of less than $1 USD per extraction, this method provides an economical, rapid, and effective method to recover HSV-1/2 DNA from swabs suitable for real-time HSV PCR. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：261 / 264
页数：4
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