Characterization of a new &ITPm2 &ITallele associated with broad-spectrum powdery mildew resistance in wheat line Subtil

被引：11
作者：
Jin, Yuli [1 ,3 ]
Xu, Hongxing [1 ]
Ma, Pengtao [1 ]
Fu, Xiaoyi [2 ]
Song, Liping [1 ]
Xu, Yunfeng [1 ]
Zhang, Xiaotian [1 ]
An, Diaoguo [1 ]
机构：
[1] Chinese Acad Sci, Ctr Agr Resources Res, Inst Genet & Dev Biol, Shijiazhuang 050021, Hebei, Peoples R China
[2] Shijiazhuang Acad Agr & Forestry Sci, Shijiazhuang 050041, Hebei, Peoples R China
[3] Univ Chinese Acad Sci, Coll Life Sci, Beijing 101408, Peoples R China
来源：
SCIENTIFIC REPORTS | 2018年 / 8卷
基金：
中国国家自然科学基金;
关键词：
MARKER-ASSISTED SELECTION; GENE; IDENTIFICATION; MAP; PM2; LOCUS; GENERATION; LINKAGE; ALLELE;
D O I：
10.1038/s41598-017-18827-4
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Wheat powdery mildew is a severe disease affecting yield and quality. Host resistance was proved to be effective and environment-friendly. Wheat line Subtil is an elite germplasm resource resistant to 28 of 30 tested Bgt isolates. Genetic analysis showed that the powdery mildew resistance in Subtil was conferred by a single dominant gene, temporarily designated PmSub. Using bulked segregant analysis, PmSub was mapped to chromosome arm 5DS, and flanked by the markers Bwm16 and Cfc181/Bwm21 at 5.0 and 0.9 cM, respectively. Allelism tests further confirmed PmSub was allelic with documented Pm2 alleles. Then, homologous sequences of Pm2a related sequence was cloned from Subtil and Chinese Spring. It was completely identical to the reported Pm2a sequence, but significantly different from that of Chinese Spring. A marker SWGI067 was developed based on the sequence divergence of homologous sequence in Subtil and Chinese Spring. SWGI067 was closely linked to PmSub, indicating that the gene PmSub itself was different from the cloned Pm2a related sequence. Meanwhile, Subtil produced significantly different reaction pattern compared with other genotypes with Pm genes at or near Pm2 locus. Therefore, PmSub was most likely a new allele of Pm2. PmSub has opportunities for marker-assisted selecting for high-efficiency wheat improvement.
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页数：9
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