Green Immobilization of Glucanobacter xylinum onto Natural Polymers to Sustainable Bacterial Cellulose Production

Bacterial cellulose (BC) has profound applications in different sectors of biotechnology due to its unique properties which made it preferring it about plant cellulose. Although this polymer is extremely important in various applications, many problems still hinder the sustainable production in terms of increasing productivity and low-cost production. In order to overcome these problems, the continuous production potentiality of cellulose using immobilized Glucanobacter xylinum cells onto Sugar cane bagasse (SCB) and Ca-alginate beads will be evaluated. Comparatively, adsorption of Glucanobacter xylinum cells to the cavum of stalk cells of SCB could be efficiently stable while, entrapment of cells onto Ca-alginate has drawback observed by the rapid disruption and instability of the beads in the Potato Peel Waste (PPW) culture medium. Furthermore, the FT-IR, XRD and SEM analysis of the BC derived from immobilized cells on SCB observed a higher crystallinity (86%) than that produced from immobilized cells on alginate beads. Consequently, BC production was statistically optimized by SCB-immobilized cells using Plackett-Burman Design. Among seven selected variables, incubation period and pH value were found to be the highest significant parameters. Reusability of immobilized biomass was studied and showed continuous BC production even after five cycles without losing their activity. Our findings demonstrate that a combination between alternative low-cost medium with continuous production mode by immobilization onto inexpensive natural polymer can promote a sustainable bioprocess and reduction the production cost. [GRAPHICS] .