MECHANISM OF M2 MACROPHAGES PROMOTING MIGRATION, INVASION AND DRUG RESISTANCE OF GASTRIC CANCER CELLS THROUGH EXOSOMES

被引：0

作者：

Li, Yandong ^{[1
]}

Zuo, Jing ^{[2
]}

Ren, Chunhong ^{[3
]}

Du, Peige ^{[1
]}

An, Liping ^{[1
]}

Gu, Xiao ^{[1
]}

Zhang, Chunyang ^{[1
]}

Dong, Xiaoguang ^{[1
]}

Ding, Xiyan ^{[1
]}

机构：

[1] Beihua Univ, Jilin 132011, Jilin, Peoples R China

[2] Zuojia Town Cent Hlth Ctr, Jilin 132109, Jilin, Peoples R China

[3] Siping Women & Infant Hosp, Siping City 136000, Jilin, Peoples R China

来源：

ACTA MEDICA MEDITERRANEA | 2022年 / 38卷 / 01期

关键词：

Migration; invasion; and drug resistance of M2 macrophages; exosomes; and gastric cancer cells; TUMOR-ASSOCIATED MACROPHAGES;

D O I：

10.19193/0393-6384_2022_1_23

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective: To explore the mechanism of M2 macrophages promoting the migration, invasion and drug resistance of gastric cancer cells through exosome transmission. Method: The gastric cancer cell line MFC and mouse bone marrow-derived macrophages were cultured to construct the macrophage polarization model in vitro. The cells were divided into three groups; the M2 macrophage group, the M2 macrophage exosomes group, and the control group. The control group and miR-21 overexpression group were obtained after culture. The cell survival rate, apoptosis and the expression of PTEN, PI3K, and Akt were measured. Results: Compared with the control + cisplatin group, the cell survival rate of the M2 macrophage + cisplatin group and the M2 macrophage exosome + cisplatin group were significantly increased, and the apoptosis rate was significantly decreased (P < 0.05), and the cell survival rate of the M2 macrophage exosome + cisplatin group was significantly higher than that of the M2 macrophage + cisplatin group, and the apoptosis rate was significantly lower than that of the M2 macrophage + cisplatin group (P < 0.05). Compared with the control group, the cell survival rate, PI3K, and Akt expression levels were significantly increased in the miR-21 overexpression group, while the apoptosis rate and PTEN expression level were significantly decreased (P < 0.05). Conclusion: M2 macrophages can transfer apoE through exosomes, activate the PI3K/Akt signaling pathway, and enhance the invasion and migration ability of gastric cancer cells. In addition, miR-21 in exosomes of M2 macrophages can be transferred to gastric cancer cells through exosomes, inhibit PTEN expression, and activate the PI3K/Akt signaling pathway, thus enhancing their resistance to cisplatin.

引用

页码：149 / 154

页数：6

共 15 条

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