Long Noncoding RNA C21orF96 Promotes the Migration, Invasion and Lymph Node Metastasis in Gastric Cancer

被引：13

作者：

Yang, Ziguo ^{[1
]}

Zhi, Qiaoming ^{[2
]}

Wang, Dan ^{[3
]}

Zhang, Li ^{[1
]}

Preston, Burnley ^{[4
]}

Brandon, Coder ^{[4
]}

Kuang, Yuting ^{[2
]}

Miao, Ruizhen ^{[1
]}

Shi, Yulong ^{[1
]}

Guo, Xiaobo ^{[1
]}

机构：

[1] Shandong Univ, Shandong Prov Hosp, Dept Gastrointestinal Surg, Jinan 250021, Peoples R China

[2] Soochow Univ, Affiliated Hosp 1, Dept Gen Surg, Suzhou 215000, Peoples R China

[3] Shandong Univ, Shandong Prov Hosp, Dept Sci & Educ, Jinan 250021, Peoples R China

[4] Univ North Texas Hlth Sci Ctr Ft Worth, Dept Cell Biol & Immunol, Ft Worth, TX 76107 USA

来源：

ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY | 2016年 / 16卷 / 09期

基金：

中国博士后科学基金;

关键词：

Lymph node; gastric cancer; lncRNA; microarray; C21orf96; TUMOR-SUPPRESSOR; EXPRESSION; CARCINOMA; HOTAIR; LYMPHANGIOGENESIS; SURVIVAL; CELLS; CYCLOOXYGENASE-2; TRANSCRIPTION; PROGNOSIS;

D O I：

10.2174/1871520616666151116122921

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Lymphatic metastasis is a primary cause of gastric cancer-related death, yet factors governing tumor cell lymphatic metastasis have not been fully elucidated. Little is known about the contributions of long noncoding RNAs (lncRNAs) to lymphatic metastasis in gastric cancer. Differentially expressional lncRNAs between metastatic lymph node tissues and normal lymph node tissues were identified and validated by microarray and quantitative real-time polymerase chain reaction (qRT-PCR), respectively. Our results found that the expression level of C21orF96 was over-expressed in positive lymph node tissues and gastric cancer tissues. We evaluated the altered expressions of C21orF96 in gastric cancer tissues comparing to adjacent normal specimens, and their association with clinicopathological factors. We showed that the expression levels of C21orF96 were associated with gross appearance, lymphatic metastasis and distal metastasis. The effect of C21orF96 was assessed by over-expressing the lncRNA. We also found that C21orF96 promoted the tubular formation, migration and invasion. Together, our results suggest that C21orF96 is an oncogenic lncRNA that promotes tumor progression and plays a pivotal role in the development of gastric cancer.

引用

页码：1101 / 1108

页数：8

共 30 条

[1] Analysis of long non-coding RNA expression profiles in gastric cancer [J].

Cao, Wei-Jun ;

Wu, Hai-Lu ;

He, Bang-Shun ;

Zhang, Yu-Shu ;

Zhang, Zhen-Yu .

WORLD JOURNAL OF GASTROENTEROLOGY, 2013, 19 (23) :3658-3664

[2] Expression of cyclooxygenase-2 and vascular endothelial growth factor-C correlates with lymphangiogenesis and lymphatic invasion in human gastric cancer [J].

Da, Ming-Xu ;

Wu, Xiao-Ting ;

Wang, Jie ;

Guo, Tian-Kang ;

Zhao, Zi-Gang ;

Luo, Ting ;

Zhang, Ming-Ming ;

Qian, Kun .

ARCHIVES OF MEDICAL RESEARCH, 2008, 39 (01) :92-99

[3] Enhanced Expression of Long Non-Coding RNA HOTAIR Is Associated with the Development of Gastric Cancer [J].

Endo, Hiroyuki ;

Shiroki, Takeharu ;

Nakagawa, Takayuki ;

Yokoyama, Misa ;

Tamai, Keiichi ;

Yamanami, Hideaki ;

Fujiya, Tsuneaki ;

Sato, Ikuro ;

Yamaguchi, Kazunori ;

Tanaka, Nobuyuki ;

Iijima, Katsunori ;

Shimosegawa, Tooru ;

Sugamura, Kazuo ;

Satoh, Kennichi .

PLOS ONE, 2013, 8 (10)

[4] Transcribed ultraconserved noncoding RNAs (T-UCR) are involved in Barrett's esophagus carcinogenesis [J].

Fassan, Matteo ;

Dall'Olmo, Luigi ;

Galasso, Marco ;

Braconi, Chiara ;

Pizzi, Marco ;

Realdon, Stefano ;

Volinia, Stefano ;

Valeri, Nicola ;

Gasparini, Pierluigi ;

Baffa, Raffaele ;

Souza, Rhonda F. ;

Vicentini, Caterina ;

D'Angelo, Edoardo ;

Bornschein, Jan ;

Nuovo, Gerard J. ;

Zaninotto, Giovanni ;

Croce, Carlo M. ;

Rugge, Massimo .

ONCOTARGET, 2014, 5 (16) :7162-7171

[5] Lymphangiogenesis in gastric carcinoma correlates with prognosis [J].

Gao, Peng ;

Zhou, Geng-Yin ;

Zhang, Qing-Hui ;

Su, Zhong-Xue ;

Zhang, Ting-Guo ;

Xiang, Lei ;

Wang, Yan ;

Zhang, Shun-Li ;

Mu, Kun .

JOURNAL OF PATHOLOGY, 2009, 218 (02) :192-200

[6] The many faces of long noncoding RNAs [J].

Gardini, Alessandro ;

Shiekhattar, Ramin .

FEBS JOURNAL, 2015, 282 (09) :1647-1657

[7] Homeobox gene IRX1 is a tumor suppressor gene in gastric carcinoma [J].

Guo, X. ;

Liu, W. ;

Pan, Y. ;

Ni, P. ;

Ji, J. ;

Guo, L. ;

Zhang, J. ;

Wu, J. ;

Jiang, J. ;

Chen, X. ;

Cai, Q. ;

Li, J. ;

Zhang, J. ;

Gu, Q. ;

Liu, B. ;

Zhu, Z. ;

Yu, Y. .

ONCOGENE, 2010, 29 (27) :3908-3920

[8] Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis [J].

Gupta, Rajnish A. ;

Shah, Nilay ;

Wang, Kevin C. ;

Kim, Jeewon ;

Horlings, Hugo M. ;

Wong, David J. ;

Tsai, Miao-Chih ;

Hung, Tiffany ;

Argani, Pedram ;

Rinn, John L. ;

Wang, Yulei ;

Brzoska, Pius ;

Kong, Benjamin ;

Li, Rui ;

West, Robert B. ;

van de Vijver, Marc J. ;

Sukumar, Saraswati ;

Chang, Howard Y. .

NATURE, 2010, 464 (7291) :1071-U148

[9] Inhibition of cyclooxygenase-2 suppresses lymph node metastasis via reduction of lymphangiogenesis [J].

Iwata, Caname ;

Kano, Mitsunobu R. ;

Komuro, Akiyoshi ;

Oka, Masako ;

Kiyono, Kunihiko ;

Johansson, Erik ;

Morishita, Yasuyuki ;

Yashiro, Masakazu ;

Hirakawa, Kosei ;

Kaminishi, Michio ;

Miyazono, Kohei .

CANCER RESEARCH, 2007, 67 (21) :10181-10189

[10] MALAT-1, a novel noncoding RNA, and thymosin β4 predict metastasis and survival in early-stage non-small cell lung cancer [J].

Ji, P ;

Diederichs, S ;

Wang, WB ;

Böing, S ;

Metzger, R ;

Schneider, PM ;

Tidow, N ;

Brandt, B ;

Buerger, H ;

Bulk, E ;

Thomas, M ;

Berdel, WE ;

Serve, H ;

Müller-Tidow, C .

ONCOGENE, 2003, 22 (39) :8031-8041

← 1 2 3 →