Structure of a neutral exopolysaccharide produced by Lactobacillus delbrueckii ssp bulgaricus LBB.B26

被引:21
作者
Sanchez-Medina, Inmaculada
Gerwig, Gerrit J.
Urshev, Zoltan L.
Kamerling, Johannis P.
机构
[1] Univ Utrecht, Bijvoet Ctr, Dept Bio Organ Chem, NL-3584 CH Utrecht, Netherlands
[2] R&D Ctr, LB Bulgaricum Plc, Sofia 1202, Bulgaria
关键词
Lactobacillus delbrueckii spp. bulgaricus; exopolysaccharide; lactic acid bacteria; structural analysis; NMR spectroscopy; mass spectrometry;
D O I
10.1016/j.carres.2007.06.014
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The neutral exopolysaccharide produced by Lactobacillus delbrueckii ssp. bulgaricus LBB.B26 in skimmed milk was found to be composed Of D-glucose and D-galactose in a molar ratio of 2:3. Linkage analysis and 1D/2D NMR (H-1 and C-13) studies performed on the native polysaccharide, and on an oligosaccharide obtained from a partial acid hydrolysate of the native polysaccharide, showed the polysaccharide to consist of branched pentasaccharide repeating units with the following structure: [GRAPHICS] (C) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2430 / 2439
页数:10
相关论文
共 50 条
  • [41] DETERMINATION OF THE STRUCTURE OF THE EXOPOLYSACCHARIDE PRODUCED BY LACTOBACILLUS-SAKE-0-1
    ROBIJN, GW
    VANDENBERG, DJC
    HAAS, H
    KAMERLING, JP
    VLIEGENTHART, JFG
    [J]. CARBOHYDRATE RESEARCH, 1995, 276 (01) : 117 - 136
  • [42] A novel method for the detection of protease and the development of extracellular protease in early growth stages of Lactobacillus delbrueckii ssp. bulgaricus
    Kawai, Y
    Tadokoro, K
    Konomi, R
    Itoh, K
    Saito, T
    Kitazawa, H
    Itoh, T
    [J]. JOURNAL OF DAIRY SCIENCE, 1999, 82 (03) : 481 - 485
  • [43] Antimicrobial activity and antibiotic resistance of Lactobacillus delbrueckii ssp bulgaricus and Streptococcus thermophilus strains isolated from Turkish homemade yoghurts
    Akpinar, Asli
    Yerlikaya, Oktay
    Kilic, Sevda
    [J]. AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH, 2011, 5 (06): : 675 - 682
  • [44] Viability of Streptococcus thermophilus, Lactobacillus delbrueckii ssp bulgaricus, Lactobacillus acidophilus and Lactobacillus casei in fermented milk supplemented with isomalto-oligosaccharides derived from banana flour
    Chockchaisawasdee, Suwimol
    Stathopoulos, Costas E.
    [J]. JOURNAL OF FOOD AND NUTRITION RESEARCH, 2011, 50 (02): : 125 - 132
  • [45] Cryoprotectants lead to phenotypic adaptation to freeze-thaw stress in Lactobacillus delbrueckii ssp bulgaricus CIP 101027T
    Panoff, JM
    Thammavongs, B
    Guéguen, M
    [J]. CRYOBIOLOGY, 2000, 40 (03) : 264 - 269
  • [46] Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp. bulgaricus in skimmed milk
    Yang, Yue
    Zhou, Wenxia
    Yang, Lingyu
    Chen, Yilun
    Sun-Waterhouse, Dongxiao
    Li, Dapeng
    [J]. FOOD SCIENCE AND HUMAN WELLNESS, 2024, 13 (05) : 3018 - 3030
  • [47] In vitro digestion and fermentation of released exopolysaccharides (r-EPS) from Lactobacillus delbrueckii ssp. bulgaricus SRFM-1
    Tang, Weizhi
    Zhou, Jianzhong
    Xu, Qian
    Dong, Mingsheng
    Fan, Xia
    Rui, Xin
    Zhang, Qiuqin
    Chen, Xiaohong
    Jiang, Mei
    Wu, Junjun
    Li, Wei
    [J]. CARBOHYDRATE POLYMERS, 2020, 230
  • [48] Influence of addition of proteolytic strains of Lactobacillus delbrueckii subsp bulgaricus to commercial ABT starter cultures on texture of yoghurt, exopolysaccharide production and survival of bacteria
    Shihata, A
    Shah, NP
    [J]. INTERNATIONAL DAIRY JOURNAL, 2002, 12 (09) : 765 - 772
  • [49] Structure and functions of exopolysaccharide produced by gut commensal Lactobacillus reuteri 100-23
    Sims, Ian M.
    Frese, Steven A.
    Walter, Jens
    Loach, Diane
    Wilson, Michelle
    Appleyard, Kay
    Eason, Jocelyn
    Livingston, Megan
    Baird, Margaret
    Cook, Gregory
    Tannock, Gerald W.
    [J]. ISME JOURNAL, 2011, 5 (07) : 1115 - 1124
  • [50] Structure of a viscous exopolysaccharide produced by Lactobacillus helveticus K16
    Yang, ZN
    Staaf, M
    Huttunen, E
    Widmalm, G
    [J]. CARBOHYDRATE RESEARCH, 2000, 329 (02) : 465 - 469