A group-specific, quantitative real-time PCR assay for detection of crab, a crustacean shellfish allergen, in complex food matrices

被引：27

作者：

Eischeid, Anne C. ^{[1
]}

Stadig, Sarah R. ^{[1
]}

机构：

[1] US FDA, Ctr Food Safety & Appl Nutr, Off Regulatory Sci, 5100 Paint Branch Pkwy, College Pk, MD 20740 USA

来源：

FOOD CHEMISTRY | 2018年 / 244卷

关键词：

Crab; Crustacean; qPCR; Real-time PCR; Allergen; Mitochondria; 12S rRNA gene; Internal amplification control; DNA; PRESSURE;

D O I：

10.1016/j.foodchem.2017.10.045

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A real-time PCR assay was developed for detection of crab, a crustacean allergen, in food products. Group-specific primers and probes were developed to detect numerous species of crab. Method validation included tests of detection in complex food matrices, evaluation of commercial food products, and cross-reactivity testing on a wide variety of crustaceans. The method was able to detect several species of crab spiked into complex food matrices at levels ranging from 0.1 to 10(5) parts per million (weight/weight), worked equally well on different platforms, exhibited high specificity for crab over other types of crustaceans, and yielded much higher signals from commercial food products listing crab as an ingredient than from those containing other crustaceans.

引用

页码：224 / 231

页数：8

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