Increased oxidative stress and activated heat shock proteins in human cell lines by silver nanoparticles

被引：42
作者：
Xin, L. [1 ]
Wang, J. [2 ]
Wu, Y. [1 ]
Guo, S. [1 ]
Tong, J. [1 ]
机构：
[1] Soochow Univ, Coll Med, Sch Publ Hlth, Suzhou 215123, Peoples R China
[2] KunShan Hlth Inspect Stn, Kunshan, Jiangsu, Peoples R China
来源：
HUMAN & EXPERIMENTAL TOXICOLOGY | 2015年 / 34卷 / 03期
基金：
中国博士后科学基金;
关键词：
Silver nanoparticles; oxidative damage; HSPAIA; HO-1; HEME OXYGENASE-1; HEAT-SHOCK-PROTEIN-70; TOXICITY; PATHWAY; CHAPERONES; EXPRESSION; INDUCTION; APOPTOSIS; EXPOSURE; MONOXIDE;
D O I：
10.1177/0960327114538988
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
Due to widely commercial applications of silver nanoparticles (Ag NPs), toxicity assessment of this NP is of great importance. This study aimed to investigate the oxidative stress and heat shock response of Ag NPs at different doses to A549 and HepG2 cells. After treatment with different concentrations of Ag NPs for 24 h, oxidative damage indicated by nnalondialdehyde (MDA), 8-epi-PGF2 alpha, and 8-hydroxy-2'-deoxyguanosine (8-oxo-dG) concentrations and protein levels of heat shock protein AIA (HSPAIA) and henne oxygenase 1 (HO-1) were determined. Ag NPs induced dose-dependent increases in MDA, 8-epi-PGF2a, and 8-oxo-dG concentrations in both A549 and HepG2 cells. Stress-inducible HSPAIA and HO-1 were also significantly upregulated in a dose-dependent manner. A higher level of HSPAIA and HO-1 activation by Ag NPs occurred in HepG2 cells than that in A549 cells. Compared with that of HSPAIA, Ag NPs induced a stronger increase in protein level of HO-1 in both cell lines. Significant positive correlations between protein levels of HSPAIA and HO-1 and oxidative damage were also observed. In conclusion, Ag NPs could induce oxidative stress in human cell lines. In addition to the products of oxidative stress such as MDA and 8-oxo-dG, HSPs can be used as potential biomarkers in nanotoxicity assessment, especially HO-1.
引用
收藏
页码：315 / 323
页数：9
相关论文
共 40 条
[1]   Silver nanoparticles induced heat shock protein 70, oxidative stress and apoptosis in Drosophila melanogaster [J].
Ahamed, Maqusood ;
Posgai, Ryan ;
Gorey, Timothy J. ;
Nielsen, Mark ;
Hussain, Saber M. ;
Rowe, John J. .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2010, 242 (03) :263-269
[2]   Physiological responses and expression of metallothionein (MT) and superoxide dismutase (SOD) rnRNAs in olive flounder, Paralichthys olivaceus exposed to benzo[a]pyrene [J].
An, Kwang Wook ;
Shin, Hyun Suk ;
Choi, Cheol Young .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2008, 149 (03) :534-539
[3]   Silver nanoparticles induce toxicity in A549 cells via ROS-dependent and ROS-independent pathways [J].
Chairuangkitti, Porntipa ;
Lawanprasert, Somsong ;
Roytrakul, Sittiruk ;
Aueviriyavit, Sasitorn ;
Phummiratch, Duangkamol ;
Kulthong, Kornphimol ;
Chanvorachote, Pithi ;
Maniratanachote, Rawiwan .
TOXICOLOGY IN VITRO, 2013, 27 (01) :330-338
[4]   RAPID COLORIMETRIC ASSAY FOR CELL-GROWTH AND SURVIVAL - MODIFICATIONS TO THE TETRAZOLIUM DYE PROCEDURE GIVING IMPROVED SENSITIVITY AND RELIABILITY [J].
DENIZOT, F ;
LANG, R .
JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 89 (02) :271-277
[5]   Genotoxicity of silver and titanium dioxide nanoparticles in bone marrow cells of rats in vivo [J].
Dobrzynska, Malgorzata M. ;
Gajowik, Aneta ;
Radzikowska, Joanna ;
Lankoff, Anna ;
Dusinska, Maria ;
Kruszewski, Marcin .
TOXICOLOGY, 2014, 315 :86-91
[6]   PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes [J].
Foldbjerg, Rasmus ;
Olesen, Ping ;
Hougaard, Mads ;
Dang, Duy Anh ;
Hoffmann, Hans Juergen ;
Autrup, Herman .
TOXICOLOGY LETTERS, 2009, 190 (02) :156-162
[7]   Effects of Silver Nanoparticles on the Liver and Hepatocytes In Vitro [J].
Gaiser, Birgit K. ;
Hirn, Stephanie ;
Kermanizadeh, Ali ;
Kanase, Nilesh ;
Fytianos, Kleanthis ;
Wenk, Alexander ;
Haberl, Nadine ;
Brunelli, Andrea ;
Kreyling, Wolfgang G. ;
Stone, Vicki .
TOXICOLOGICAL SCIENCES, 2013, 131 (02) :537-547
[8]   How the doors to the nanoworld were opened [J].
Gerber, Christoph ;
Lang, Hans Peter .
NATURE NANOTECHNOLOGY, 2006, 1 (01) :3-5
[9]   Benzo(a)pyrene inhibits expression of inducible heat shock protein 70 in vascular endothelial cells [J].
Gong, Z. ;
Yang, J. ;
Yang, M. ;
Wang, F. ;
Wei, Q. ;
Tanguay, R. M. ;
Wu, T. .
TOXICOLOGY LETTERS, 2006, 166 (03) :229-236
[10]   Mechanisms of Cell Protection by Heme Oxygenase-1 [J].
Gozzelino, Raffaella ;
Jeney, Viktoria ;
Soares, Miguel P. .
ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 2010, 50 :323-354
1234