PREVALENCE AND GENOTYPING OF CRYPTOSPORIDIUM SPP FROM DAIRY COW FECAL SAMPLES IN WESTERN THAILAND

被引：1

作者：

Inpankaew, Tawin ^{[1
]}

Jiyipong, Tawisa ^{[1
]}

Pinyopanuwat, Nongnuch ^{[1
]}

Chimnoi, Wissanuwat ^{[1
]}

Thompson, R. C. Andrew ^{[2
]}

Jittapalapong, Sathaporn ^{[1
]}

机构：

[1] Kasetsart Univ, Fac Vet Med, Dept Parasitol, Bangkok 10900, Thailand

[2] Murdoch Univ, Sch Vet & Biomed Sci, WHO Collaborating Ctr Mol Epidemiol Parasit Infec, Murdoch, WA 6150, Australia

来源：

SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH | 2010年 / 41卷 / 04期

关键词：

Cryptosporidium; dairy cows; acid-fast staining; PCR-RFLP; Thailand; HIV-INFECTED PATIENTS; SP APICOMPLEXA; GIARDIA; CATTLE; PARVUM; CALVES;

D O I：

暂无

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The aims of this study were to determine the prevalence of Cryptosporidium spp in dairy cows in central Thailand and to investigate the genotype of Cryptosporidium spp in this population. A total of 200 fecal samples from dairy cows were collected and examined by the acid-fast staining technique and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The prevalence of Cryptosporidium infection in dairy cows was 7% (95%CI 3.5-10.5) by acid-fast staining, and 15.5% (95%CI 10.5-20.5) by PCR-RFLR This is the first report of genetic identification of the C. parvum bovine genotype in dairy cows in Thailand. PCR-RFLP analysis showed all positive samples were C. parvum (bovine genotype). C. andersoni was not found in this study. The only significant risk factor for Cryptosporidium infection in dairy cows was age. Calves less than 2 months old were more frequently infected by Cryptosporidium than others (OR 13.82, 95%CI 3.67-51.97, p=0.001). Cattle may be a potential source of human cryptosporidiosis.

引用

页码：770 / 775

页数：6

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