Structure and properties of pectin can be affected by extraction methods. In this study, grapefruit peel pectins extracted by HCl (at pH 1 [P1], 2 [P2], and 3 [P3]) and NaOH (at pH 9 [P9], 10 [P10], and 11 [P11]) were prepared and characterized. Atomic force microscopy (AFM) provided direct evidence of complex nano-structural patterns of pectins and revealed cross-linked networks of P10 and P11. Small-angle X-ray scattering (SAXS) demonstrated that P1, P2, and P3 possessed a relatively extended conformation, whereas P9, P10, and P11 displayed a three-dimensional structure and folded conformation. The compact and extended conformations of P3 contributed to its high viscosity in solution and the stability of the formed emulsion (75%). Porous surface and larger three-dimensional nanostructure (D-max: 23 nm) of P10 facilitated its ion-binding capacity. Our results provide valuable insight into relationship between extraction methods and structure-properties of pectin, facilitating design of functional pectins.
机构:
Hauptman Woodward Med Res Inst, Buffalo, NY 14203 USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
Grant, Thomas D.
;
Luft, Joseph R.
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机构:
Hauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
SUNY Buffalo, Dept Struct & Computat Biol, Buffalo, NY 14203 USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
Luft, Joseph R.
;
Wolfley, Jennifer R.
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h-index: 0
机构:
Hauptman Woodward Med Res Inst, Buffalo, NY 14203 USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
Wolfley, Jennifer R.
;
Tsuruta, Hiro
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机构:
SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, Menlo Pk, CA 94025 USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
Tsuruta, Hiro
;
Martel, Anne
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机构:
SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, Menlo Pk, CA 94025 USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
Martel, Anne
;
Montelione, Gaetano T.
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机构:
Rutgers State Univ, Ctr Adv Biotechnol & Med, Dept Mol Biol & Biochem, NE Struct Genom Consortium, Piscataway, NJ 08854 USA
Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Biochem, Piscataway, NJ USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
Montelione, Gaetano T.
;
Snell, Edward H.
论文数: 0引用数: 0
h-index: 0
机构:
Hauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
SUNY Buffalo, Dept Struct & Computat Biol, Buffalo, NY 14203 USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
机构:
Hauptman Woodward Med Res Inst, Buffalo, NY 14203 USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
Grant, Thomas D.
;
Luft, Joseph R.
论文数: 0引用数: 0
h-index: 0
机构:
Hauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
SUNY Buffalo, Dept Struct & Computat Biol, Buffalo, NY 14203 USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
Luft, Joseph R.
;
Wolfley, Jennifer R.
论文数: 0引用数: 0
h-index: 0
机构:
Hauptman Woodward Med Res Inst, Buffalo, NY 14203 USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
Wolfley, Jennifer R.
;
Tsuruta, Hiro
论文数: 0引用数: 0
h-index: 0
机构:
SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, Menlo Pk, CA 94025 USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
Tsuruta, Hiro
;
Martel, Anne
论文数: 0引用数: 0
h-index: 0
机构:
SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, Menlo Pk, CA 94025 USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
Martel, Anne
;
Montelione, Gaetano T.
论文数: 0引用数: 0
h-index: 0
机构:
Rutgers State Univ, Ctr Adv Biotechnol & Med, Dept Mol Biol & Biochem, NE Struct Genom Consortium, Piscataway, NJ 08854 USA
Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Biochem, Piscataway, NJ USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
Montelione, Gaetano T.
;
Snell, Edward H.
论文数: 0引用数: 0
h-index: 0
机构:
Hauptman Woodward Med Res Inst, Buffalo, NY 14203 USA
SUNY Buffalo, Dept Struct & Computat Biol, Buffalo, NY 14203 USAHauptman Woodward Med Res Inst, Buffalo, NY 14203 USA