Sulfation of nod factors via nodHPQ is nodD independent in Rhizobium tropici CIAT899

被引：4
|
作者：
Folch-Mallol, JL
Manyani, H
Marroquí, S
Sousa, C
Vargas, C
Nava, N
Colmenero-Flores, JM
Quinto, A
Megias, M
机构：
[1] Univ Seville, Fac Pharm, Dept Microbiol & Parasitol, E-41012 Seville, Spain
[2] Univ Nacl Autonoma Mexico, Inst Biotechnol, Dept Plant Mol Biol, Cuernavaca 62271, Morelos, Mexico
来源：
MOLECULAR PLANT-MICROBE INTERACTIONS | 1998年 / 11卷 / 10期
关键词：
D O I：
10.1094/MPMI.1998.11.10.979
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A cosmid from the Rhizobium tropici CIAT899 symbiotic plasmid, containing most of the nodulation genes described in this strain, has been isolated. Although this cosmid does not carry a nodD gene, it confers ability to heterologous Rhizobium spp, to nodulate R. tropici hosts (Phaseolus vulgaris, Macroptilium atropurpureum, and Leucaena leucocephala). The observed phenotype is due to constitutive expression of the nodABCSUIJ operon, which has lost its regulatory region and is expressed from a promoter present in the cloning vector. Thin-layer chromatography (TLC) analysis of the Nod factors produced by this construction shows that it is still capable of synthesizing sulfated compounds, suggesting that the nodHPQ genes are organized as an operon that is transcribed in a nodD-independent manner and is not regulated by flavonoids.
引用
收藏
页码：979 / 987
页数：9
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